{"doi":"10.7171/jbt.17-2801-009","title":"Real-Time DNA Sequencing in the Antarctic Dry Valleys Using the Oxford Nanopore Sequencer","abstract":null,"journal":"Journal of Biomolecular Techniques : JBT","year":2017,"id":629207,"datarank":0.7667981682534817,"base_score":5.111987788356544,"endowment":5.111987788356544,"self_citation_contribution":0.7667981682534817,"citation_network_contribution":0.0,"self_endowment_contribution":0.7667981682534817,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":165,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1201270,"name":"Elena Zaikova","orcid":"0000-0002-2422-3108","position":1,"is_corresponding":false},{"id":1629412,"name":"David S. Goerlitz","orcid":null,"position":2,"is_corresponding":false},{"id":480418,"name":"Yu Bai","orcid":"0000-0003-0515-8248","position":3,"is_corresponding":false},{"id":1629413,"name":"Scott W. Tighe","orcid":null,"position":4,"is_corresponding":false},{"id":1629411,"name":"Sarah S. Johnson","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Real-Time DNA Sequencing in the Antarctic Dry Valleys Using the Oxford Nanopore Sequencer","abstract":"The ability to sequence DNA outside of the laboratory setting has enabled novel research questions to be addressed in the field in diverse areas, ranging from environmental microbiology to viral epidemics. Here, we demonstrate the application of offline DNA sequencing of environmental samples using a hand-held nanopore sequencer in a remote field location: the McMurdo Dry Valleys, Antarctica. Sequencing was performed using a MK1B MinION sequencer from Oxford Nanopore Technologies (ONT; Oxford, United Kingdom) that was equipped with software to operate without internet connectivity. One-direction (1D) genomic libraries were prepared using portable field techniques on DNA isolated from desiccated microbial mats. By adequately insulating the sequencer and laptop, it was possible to run the sequencing protocol for up to 2½ h under arduous conditions.","is_dataset_classified":null,"base_score":5.111987788356544,"endowment":5.111987788356544,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"28337073","pmcid":"PMC5362188","openalex_id":"https://openalex.org/W2602845639","authors":[],"funders":[],"total_grants":0,"fwci":17.9093,"citation_percentile":0.99637174,"influential_citations":0,"citation_trend":[{"year":2016,"count":2},{"year":2017,"count":11},{"year":2018,"count":22},{"year":2019,"count":33},{"year":2020,"count":24},{"year":2021,"count":21},{"year":2022,"count":11},{"year":2023,"count":23},{"year":2024,"count":9},{"year":2025,"count":8},{"year":2026,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362188/pdf/jbt.28-2.pdf","host_type":"journal"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362188/pdf/jbt.28-2.pdf","host_type":"publisher"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5362188/","host_type":"publisher"},{"url":"https://doi.org/10.7171/jbt.17-2801-009","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/28337073","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/5362188","host_type":"repository"},{"url":"http://doi.org/10.7171/jbt.17-2801-009","host_type":"repository"}],"fields_of_study":["Bacteriophages and microbial interactions","Genomics and Phylogenetic Studies","Polar Research and Ecology"],"mesh_terms":["Antarctic Regions","Desert Climate","Environmental Microbiology","Bacteriophage lambda","Reference Standards","Sequence Analysis, DNA","Molecular Typing","High-Throughput Nucleotide Sequencing"],"keywords":["Minion","Nanopore sequencing","DNA sequencer","DNA sequencing","Nanopore","Laptop","Computer science","Computational biology","Biology","DNA","Genetics","Nanotechnology","Operating system","Antarctica","Extremophiles"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-05T17:32:16.657041Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}