{"doi":"10.7171/3fc1f5fe.d13e7666","title":"Improved Yield of SPRI Beads–Based Size Selection in theVery High Molecular Weight Range","abstract":"Solid-phase reversible immobilization (SPRI) remains a cornerstone of nucleic acid purification and is a key reagent for many protocols.A key aspect of an SPRI-based purification protocol is the ability to select DNAs of certain sizes by changing the properties of the binding buffer.Modification of the standard buffers used in SPRI purification is able to shift the minimum binding length required for SPRI beads to function, allowing them to specifically select DNA of high molecular weight that are required for long read sequencing.A challenge with the modified buffers has been a poorer than expected yield.We have identified that bead saturation is the likely cause of the low yield, and increasing bead fraction improves recovery, particularly for high-molecular-weight fragments.","journal":"Journal of Biomolecular Techniques JBT","year":2024,"id":475692,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9553,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":62836,"name":"Stuart S. Levine","orcid":"0000-0001-7363-562X","position":1,"is_corresponding":false},{"id":329619,"name":"Alexei Stortchevoi","orcid":"0000-0001-9916-4344","position":0,"is_corresponding":true}],"reference_count":1,"raw_metadata":null,"created_at":"2026-07-19T02:06:21.071690Z","pmid":"39239236","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}