{"doi":"10.7150/thno.69938","title":"3D culturing of human pluripotent stem cells-derived endothelial cells for vascular regeneration","abstract":"Rationale: Human induced pluripotent stem cell-derived endothelial cells can be candidates for engineering therapeutic vascular grafts. Methods: Here, we studied the role of three-dimensional culture on their characteristics and function both in vitro and in vivo. Results: We found that differentiated hPSC-EC can re-populate decellularized biomatrices; they remain viable, undergo maturation and arterial/venous specification. Human PSC-EC develop antifibrotic, vasoactive and anti-inflammatory properties during recellularization. In vivo, a robust increase in perfusion was detected at the engraftment sites after subcutaneous implantation of an hPSC-EC-laden hydrogel in rats. Histology confirmed survival and formation of capillary-like structures, suggesting the incorporation of hPSC-EC into host microvasculature. In a canine model, hiPSC-EC-seeded onto decellularised vascular segments were functional as aortic grafts. Similarly, we showed the retention and maturation of hiPSC-EC and dynamic remodelling of the vessel wall with good maintenance of vascular patency. Conclusions: A combination of hPSC-EC and biomatrices may be a promising approach to repair ischemic tissues.","journal":"Theranostics","year":2022,"id":274176,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9572,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":942706,"name":"Eleonora Zucchelli","orcid":null,"position":1,"is_corresponding":false},{"id":942707,"name":"Annamária Nemes","orcid":null,"position":2,"is_corresponding":false},{"id":245968,"name":"Zoltán Jakus","orcid":"0000-0002-6304-2369","position":3,"is_corresponding":false},{"id":942708,"name":"Kitti Ajtay","orcid":null,"position":4,"is_corresponding":false},{"id":656110,"name":"Éva Kemecsei","orcid":null,"position":5,"is_corresponding":false},{"id":942709,"name":"Gábor Kiszler","orcid":null,"position":6,"is_corresponding":false},{"id":942238,"name":"Nikolett Hegedűs","orcid":"0000-0003-1122-1872","position":7,"is_corresponding":false},{"id":942239,"name":"Krisztián Szigeti","orcid":"0000-0002-0828-9828","position":8,"is_corresponding":false},{"id":942710,"name":"I Földes","orcid":null,"position":9,"is_corresponding":false},{"id":942711,"name":"Kristóf Árvai","orcid":null,"position":10,"is_corresponding":false},{"id":942712,"name":"János P. Kósa","orcid":null,"position":11,"is_corresponding":false},{"id":726948,"name":"Krasimir Kolev","orcid":"0000-0002-5612-004X","position":12,"is_corresponding":false},{"id":726941,"name":"Erzsébet Komorowicz","orcid":"0000-0002-4860-3498","position":13,"is_corresponding":false},{"id":942240,"name":"Parasuraman Padmanabhan","orcid":"0000-0003-4112-4600","position":14,"is_corresponding":false},{"id":515230,"name":"Pál Maurovich‐Horvat","orcid":"0000-0003-0885-736X","position":15,"is_corresponding":false},{"id":724332,"name":"Edit Dósa","orcid":"0000-0003-2984-2642","position":16,"is_corresponding":false},{"id":942241,"name":"György Várady","orcid":"0000-0003-2012-9680","position":17,"is_corresponding":false},{"id":725271,"name":"Miklós Pólos","orcid":null,"position":18,"is_corresponding":false},{"id":362599,"name":"István Hartyánszky","orcid":null,"position":19,"is_corresponding":false},{"id":397062,"name":"Siân E. Harding","orcid":"0000-0002-3651-6354","position":20,"is_corresponding":false},{"id":2664,"name":"Béla Merkely","orcid":"0000-0001-6514-0723","position":21,"is_corresponding":false},{"id":942242,"name":"Domokos Máthé","orcid":"0000-0001-7343-0413","position":22,"is_corresponding":false},{"id":942243,"name":"Gábor Szabó","orcid":"0000-0001-6038-9968","position":23,"is_corresponding":false},{"id":362600,"name":"Tamás Radovits","orcid":null,"position":24,"is_corresponding":false},{"id":794104,"name":"Gábor Földes","orcid":"0000-0003-4415-352X","position":25,"is_corresponding":false},{"id":942237,"name":"Edit Gara","orcid":"0000-0003-0074-6788","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":null,"created_at":"2026-07-19T00:28:07.776043Z","pmid":"35832092","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}