{"doi":"10.7150/thno.100802","title":"Whole-body fluorescence cryotomography identifies a fast-acting, high-contrast, durable contrast agent for fluorescence-guided surgery","abstract":"Imaging of tumor-specific fluorescent contrast agents to guide tumor removal has been shown to improve outcomes and is now standard practice for some neurosurgical procedures.However, many agents require administration hours before surgery, a practical challenge, and may exhibit inconsistent concordance with contrast-enhanced MRI (CE-MRI), the current standard for diagnosing and guiding glioma removal.A fluorescent agent that accurately marks tumor shortly after administration and is otherwise similar to CE-MRI would help overcome these shortcomings.Methods: We used whole-body 3-D fluorescence cryo-imaging and co-registered CE-MRI volumes to evaluate several fluorescent contrast agent candidates for diagnostic performance and concordance with CE-MRI.Mice with brain tumors were administered a cocktail of fluorescent agent candidates and a MRI contrast agent, and then imaged with MRI and fluorescence cryo-imaging at several timepoints after administration.The high-resolution 3-D cryo-imaging volumes of the fluorescent agents were used to determine diagnostic performance metrics and correlation with CE-MRI.Results: While all agents showed positive metrics, one agent, tetramethylrhodamine conjugated to a small polyethylene glycol chain (TMR-PEG1k), outperformed the others, exhibiting minimal normal brain signal, high tumor-to-background-ratio, diagnostic accuracy, and cross-correlation to CE-MRI at all post-administration timepoints (10-90 min) and tumor lines examined.Conclusion: These favorable properties establish TMR-PEG1k as a promising candidate for surgical guidance.","journal":"Theranostics","year":2024,"id":469548,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9508,"is_data_producer":true,"deposit_databanks":{"figshare":["10.6084/m9.figshare.c.7474965.v1"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":522346,"name":"Brook K. Byrd","orcid":"0000-0003-2857-3129","position":1,"is_corresponding":false},{"id":1295401,"name":"Caleb Y. Kwon","orcid":null,"position":2,"is_corresponding":false},{"id":512553,"name":"Rendall R. Strawbridge","orcid":null,"position":3,"is_corresponding":false},{"id":377168,"name":"Kimberley S. Samkoe","orcid":"0000-0002-8234-2308","position":4,"is_corresponding":false},{"id":312695,"name":"P. Jack Hoopes","orcid":null,"position":5,"is_corresponding":false},{"id":289301,"name":"Keith D. Paulsen","orcid":"0000-0002-6692-3196","position":6,"is_corresponding":false},{"id":34011,"name":"David W. Roberts","orcid":"0000-0002-8677-3930","position":7,"is_corresponding":false},{"id":512554,"name":"Scott C. Davis","orcid":null,"position":8,"is_corresponding":false},{"id":1294984,"name":"Augustino V. Scorzo","orcid":"0000-0001-5573-5985","position":0,"is_corresponding":true}],"reference_count":1,"raw_metadata":null,"created_at":"2026-07-19T02:05:32.241298Z","pmid":"39479457","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}