{"doi":"10.64898/2026.01.27.26344979","title":"Integrating multi-omic QTLs and predictive models reveals regulatory architectures at immune related GWAS loci in CD4+ T cells","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>Functional interpretation is essential for understanding how genetic variants contribute to complex traits. Here, we identified and characterized regulatory variants in CD4+ T cells collected from 362 donors. We integrated molecular QTL mapping from single-cell RNA-seq profiles and chromatin accessibility with predicted variant effects from a deep learning model trained on chromatin accessibility data. We identified molecular features and transcription factor binding mechanisms underlying variant sharing and mediated effects across the modalities and approaches. While predicted variant effects correlated with molQTLs, only a small fraction of empirically detected molQTLs were discovered by predictive models. MolQTLs, primarily those affecting chromatin, indicated potential molecular drivers for 33% of immune-related GWAS loci, with the deep learning approach providing insights into 4.7% of GWAS loci. These results highlight the value of multi-omic data and systematic integration of empirical and predictive approaches to interpret regulatory effects of genetic variants.</jats:p>","journal":null,"year":null,"id":653926,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1050078,"name":"Samuel Ghatan","orcid":"0000-0002-8310-3448","position":1,"is_corresponding":false},{"id":646228,"name":"Sean Bankier","orcid":"0000-0001-5069-3165","position":2,"is_corresponding":false},{"id":949304,"name":"Taylor V. Thompson","orcid":"0000-0002-0378-0052","position":3,"is_corresponding":false},{"id":1706371,"name":"Kassidy Lundy-Perez","orcid":"0000-0003-2781-6205","position":4,"is_corresponding":false},{"id":585984,"name":"Masako Suzuki","orcid":"0000-0003-0605-9225","position":5,"is_corresponding":false},{"id":944407,"name":"Reanna Doña-Termine","orcid":"0000-0001-9283-0981","position":6,"is_corresponding":false},{"id":960078,"name":"Jacob Stauber","orcid":"0000-0002-9410-8527","position":7,"is_corresponding":false},{"id":23600,"name":"David Reynolds","orcid":null,"position":8,"is_corresponding":false},{"id":948569,"name":"Kathleen Rosales","orcid":null,"position":9,"is_corresponding":false},{"id":1341501,"name":"Anthony Griffen","orcid":"0009-0001-8257-7238","position":10,"is_corresponding":false},{"id":1429862,"name":"Mariko Isshiki","orcid":"0000-0002-6565-6970","position":11,"is_corresponding":false},{"id":622955,"name":"Danny Simpson","orcid":"0000-0001-9690-3527","position":12,"is_corresponding":false},{"id":1495578,"name":"Nathanael Andrews","orcid":"0000-0003-4139-2904","position":13,"is_corresponding":false},{"id":563544,"name":"Omar Ahmed","orcid":"0000-0002-9933-8508","position":14,"is_corresponding":false},{"id":1706375,"name":"Samantha Gold","orcid":null,"position":15,"is_corresponding":false},{"id":1706376,"name":"Sophia R. Ostrowiak","orcid":null,"position":16,"is_corresponding":false},{"id":1107359,"name":"Srilakshmi M. Raj","orcid":"0000-0002-4506-7028","position":17,"is_corresponding":false},{"id":280514,"name":"Sofiya Milman","orcid":"0000-0001-9247-0082","position":18,"is_corresponding":false},{"id":14836,"name":"Tuuli Lappalainen","orcid":"0000-0002-7746-8109","position":19,"is_corresponding":false},{"id":275114,"name":"John M. Greally","orcid":"0000-0001-6069-7960","position":20,"is_corresponding":false},{"id":382652,"name":"Marliette R. Matos","orcid":"0000-0003-2641-6541","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Integrating multi-omic QTLs and predictive models reveals regulatory architectures at immune related GWAS loci in CD4+ T cells","abstract":"<jats:title>Abstract</jats:title>\n                <jats:p>Functional interpretation is essential for understanding how genetic variants contribute to complex traits. Here, we identified and characterized regulatory variants in CD4+ T cells collected from 362 donors. We integrated molecular QTL mapping from single-cell RNA-seq profiles and chromatin accessibility with predicted variant effects from a deep learning model trained on chromatin accessibility data. We identified molecular features and transcription factor binding mechanisms underlying variant sharing and mediated effects across the modalities and approaches. While predicted variant effects correlated with molQTLs, only a small fraction of empirically detected molQTLs were discovered by predictive models. MolQTLs, primarily those affecting chromatin, indicated potential molecular drivers for 33% of immune-related GWAS loci, with the deep learning approach providing insights into 4.7% of GWAS loci. These results highlight the value of multi-omic data and systematic integration of empirical and predictive approaches to interpret regulatory effects of genetic variants.</jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"41646693","pmcid":null,"openalex_id":"https://openalex.org/W7126225601","authors":[],"funders":[{"funder_name":"NIA NIH HHS","grant_id":"R01 AG057422","title":null}],"total_grants":1,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"green","license":"cc-by","oa_locations":[{"url":"https://www.medrxiv.org/content/medrxiv/early/2026/01/30/2026.01.27.26344979.full.pdf","host_type":"repository"},{"url":"https://www.medrxiv.org/content/medrxiv/early/2026/01/30/2026.01.27.26344979.full.pdf","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.64898/2026.01.27.26344979","host_type":"publisher"},{"url":"https://doi.org/10.64898/2026.01.27.26344979","host_type":"repository"},{"url":"https://pubmed.ncbi.nlm.nih.gov/41646693","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/12870616","host_type":"repository"}],"fields_of_study":["Single-cell and spatial transcriptomics","Genomics and Chromatin Dynamics","Genetic Associations and Epidemiology"],"mesh_terms":[],"keywords":["Genome-wide association study","Chromatin","Quantitative trait locus","Transcription factor","Genetic association","Genetic variants","Phenotype","Genome"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-11T02:12:46.332923Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}