{"doi":"10.64898/2026.01.19.700200","title":"A family of archaeal hibernation factors that bind in tandem and protect ribosomes in dormant cells","abstract":"<jats:title>ABSTRACT</jats:title>\n                <jats:p>\n                  Under nutrient limitation or stress, ribosome hibernation factors inactivate and protect ribosomes. Although ribosome hibernation plays an important role in microbes, we lack a complete understanding of this process in archaea. Here, we identify a family of hibernation factors, which we designate as single ribosomal subunit inhibitors (SriA-SriD), from the methanogenic archaeon\n                  <jats:italic>Methanosarcina acetivorans</jats:italic>\n                  . All four\n                  <jats:italic>sri</jats:italic>\n                  genes are encoded in an operon and each Sri protein inhibits protein synthesis\n                  <jats:italic>in vitro</jats:italic>\n                  . Deletion of\n                  <jats:italic>sri</jats:italic>\n                  genes in\n                  <jats:italic>M. acetivorans</jats:italic>\n                  impaired growth recovery after prolonged stationary phase and also led to depletion of the small ribosomal subunit. Cryo-EM structures show that Sri proteins bind to the ribosome in tandem and form conserved protein-protein interfaces. Sri is broadly distributed across archaeal phyla and\n                  <jats:italic>sri</jats:italic>\n                  genes frequently co-occur. Together, these findings establish Sri proteins as a distinct group of hibernation factors that protect ribosomes during dormancy and expand our understanding of ribosome hibernation in archaea.\n                </jats:p>","journal":null,"year":null,"id":598059,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":988107,"name":"Madison C Williams","orcid":"0000-0002-8340-4443","position":1,"is_corresponding":false},{"id":805453,"name":"Yekaterina Shulgina","orcid":"0000-0001-7658-9294","position":2,"is_corresponding":false},{"id":1532314,"name":"Roan W. Kivimae","orcid":null,"position":3,"is_corresponding":false},{"id":1063976,"name":"Dipti D. Nayak","orcid":"0000-0002-3449-3419","position":4,"is_corresponding":false},{"id":17801,"name":"Jamie H. D. Cate","orcid":"0000-0001-5965-7902","position":5,"is_corresponding":false},{"id":900523,"name":"Amos J. Nissley","orcid":"0000-0003-4829-5373","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A family of archaeal hibernation factors that bind in tandem and protect ribosomes in dormant cells","abstract":"<jats:title>ABSTRACT</jats:title>\n                <jats:p>\n                  Under nutrient limitation or stress, ribosome hibernation factors inactivate and protect ribosomes. Although ribosome hibernation plays an important role in microbes, we lack a complete understanding of this process in archaea. Here, we identify a family of hibernation factors, which we designate as single ribosomal subunit inhibitors (SriA-SriD), from the methanogenic archaeon\n                  <jats:italic>Methanosarcina acetivorans</jats:italic>\n                  . All four\n                  <jats:italic>sri</jats:italic>\n                  genes are encoded in an operon and each Sri protein inhibits protein synthesis\n                  <jats:italic>in vitro</jats:italic>\n                  . Deletion of\n                  <jats:italic>sri</jats:italic>\n                  genes in\n                  <jats:italic>M. acetivorans</jats:italic>\n                  impaired growth recovery after prolonged stationary phase and also led to depletion of the small ribosomal subunit. Cryo-EM structures show that Sri proteins bind to the ribosome in tandem and form conserved protein-protein interfaces. Sri is broadly distributed across archaeal phyla and\n                  <jats:italic>sri</jats:italic>\n                  genes frequently co-occur. Together, these findings establish Sri proteins as a distinct group of hibernation factors that protect ribosomes during dormancy and expand our understanding of ribosome hibernation in archaea.\n                </jats:p>","is_dataset_classified":null,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"41867869","pmcid":null,"openalex_id":"https://openalex.org/W7124962361","authors":[],"funders":[{"funder_name":"","grant_id":"CHE-2002182","title":null},{"funder_name":"","grant_id":"5T32GM132022","title":null}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[{"year":2026,"count":1}],"oa_status":"green","license":"cc-by-nc","oa_locations":[{"url":"https://doi.org/10.64898/2026.01.19.700200","host_type":"repository"},{"url":"https://doi.org/10.64898/2026.01.19.700200","host_type":"repository"},{"url":"https://syndication.highwire.org/content/doi/10.64898/2026.01.19.700200","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/41867869","host_type":"repository"},{"url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC13004131/","host_type":"repository"}],"fields_of_study":["RNA and protein synthesis mechanisms","RNA modifications and cancer","Bacterial Genetics and Biotechnology"],"mesh_terms":[],"keywords":["Ribosome","Ribosomal RNA","Hibernation (computing)","Ribosomal protein","Operon","Gene","Protein subunit","Translation (biology)"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T14:57:42.650654Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}