{"doi":"10.64898/2025.12.24.696318","title":"TRAF6 coiled-coil domain mediates its trimerization","abstract":"Tumor necrosis factor receptor-associated factor 6 (TRAF6) is an adaptor protein that plays a critical role in innate immune signaling. TRAF6 consists of an N-terminal RING domain, five zinc fingers, a coiled-coil domain, and a C-terminal TRAF domain. Although structural and oligomeric information for coiled-coiled domains in TRAF2-5 is known, information on TRAF6's coiled-coiled domain is relatively unknown. Here, we characterized the oligomeric state of the human TRAF6 domain (residues 288-348) using size-exclusion chromatography (SEC) and size-exclusion chromatography coupled multi-angle light scattering (SEC-MALS). Both His-tagged and tagless proteins displayed molecular masses consistent with a trimer. Coiled-coil computational tools identified a heptad repeat pattern that supported the trimeric findings. AlphaFold-Multimer modelling further showed a symmetric, trimeric coiled-coiled domain with a conserved hydrophobic core. These results provide experimental support for TRAF6 coiled-coiled domain trimerization and help establish a structural framework for understanding how TRAF6 assembles into higher-order structures.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":587140,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9559,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":719453,"name":"Rui Yang","orcid":"0000-0002-9311-1265","position":1,"is_corresponding":false},{"id":285505,"name":"Qian Yin","orcid":"0000-0002-8481-150X","position":2,"is_corresponding":false},{"id":1502218,"name":"Mohammed Masum Siraj Khan","orcid":"0000-0002-7848-4821","position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:59:36.020030Z","pmid":"41509339","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}