{"doi":"10.64898/2025.12.19.695635","title":"Deletion of the polarity protein Llgl1 impairs cardiomyocyte intercalated disc integrity and permits cell cycle activity into adulthood","abstract":"ABSTRACT Background Cell polarity orchestrates spatial organization within tissues influencing cellular processes such as adhesion, proliferation, and differentiation. While the role of polarity proteins is well-established in epithelial systems, their function in mammalian cardiomyocytes remains poorly understood. Global deletion of the polarity protein, Llgl1, has been shown to impair cardiac trabeculation and disrupt cardiomyocyte junctions in zebrafish. Here, we investigate the role of Llgl1 in mammalian cardiomyocyte function. Methods We developed a mouse model whereby Llgl1 is deleted from cardiomyocytes (Llgl1 CKO ) and assessed cardiomyocyte intercalated disc integrity, cell cycle activity, cardiac function, and electrophysiology. We subjected mice to myocardial infarction to test if permanent Llgl1 deletion, or inducible Llgl1 deletion in adult cardiomyocytes (Llgl1 TamCKO ), influences the cardiac injury response. We performed RNAsequencing and in vitro knockdown and viral overexpression experiments to delineate the mechanisms by which Llgl1 influences cardiomyocyte phenotypes. Results Llgl1 CKO mice display disrupted intercalated disc integrity indicated by reduced protein density at cardiomyocyte junctions, associated with electrical conduction defects that manifest as ventricular arrhythmia. Furthermore, Llgl1 CKO mice display enhanced cardiomyocyte cell cycle activity extending into adulthood. Llgl1 CKO mice and mice whereby Llgl1 is deleted by tamoxifen induction in adulthood both show improved cardiac function and reduced scar size following myocardial infarction. Mechanistically, Llgl1 alters mechanotransduction and cell cycle pathways in the heart, and regulates proper localization of N-cadherin to the cell membrane, a process that is optimized by phosphorylation of five serine residues at the C-terminus of Llgl1. Conclusions We elucidate a key role for Llgl1 in establishing proper intercalated disc integrity in mammalian cardiomyocytes. Disruption of intercalated discs and mechanotransduction via Llgl1 knockout is associated with cardiac arrhythmia, elevated cell cycle activity and resistance to cardiac injury. These studies shed light on mechanisms underlying intercalated disc establishment and connect disrupted cardiomyocyte junctional integrity with enhanced cell cycle activity.","journal":"bioRxiv (Cold Spring Harbor Laboratory)","year":2025,"id":586774,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9566,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":382794,"name":"Michael A. Flinn","orcid":"0000-0002-8200-1693","position":1,"is_corresponding":false},{"id":728710,"name":"Amirala Bakhshian Nik","orcid":"0000-0002-1176-1628","position":2,"is_corresponding":false},{"id":742856,"name":"Anooj Arkatkar","orcid":null,"position":3,"is_corresponding":false},{"id":1050436,"name":"Caelan Moreno","orcid":null,"position":4,"is_corresponding":false},{"id":1501825,"name":"Caroline J Greenheck","orcid":null,"position":5,"is_corresponding":false},{"id":1050437,"name":"Sydney L. Buday","orcid":null,"position":6,"is_corresponding":false},{"id":990911,"name":"Kaelin A. Akins","orcid":"0000-0002-1205-7210","position":7,"is_corresponding":false},{"id":1501826,"name":"Brianna R Waack","orcid":null,"position":8,"is_corresponding":false},{"id":1501827,"name":"J. Pindaro","orcid":null,"position":9,"is_corresponding":false},{"id":727555,"name":"Alexandra L. Purdy","orcid":"0000-0003-1632-5523","position":10,"is_corresponding":false},{"id":1501828,"name":"S. -J. Li","orcid":null,"position":11,"is_corresponding":false},{"id":773579,"name":"Bradley Miller","orcid":"0000-0002-8206-4766","position":12,"is_corresponding":false},{"id":201165,"name":"Lu Han","orcid":null,"position":13,"is_corresponding":false},{"id":648450,"name":"C. Liu","orcid":"0009-0007-2420-8278","position":14,"is_corresponding":false},{"id":352954,"name":"Michaela Patterson","orcid":"0000-0002-3805-4181","position":15,"is_corresponding":false},{"id":382802,"name":"Brian A. Link","orcid":"0000-0002-7173-2642","position":16,"is_corresponding":false},{"id":382801,"name":"Caitlin C. O’Meara","orcid":"0000-0002-9212-0821","position":17,"is_corresponding":false},{"id":462418,"name":"Jerrell Lovett","orcid":null,"position":0,"is_corresponding":true}],"reference_count":64,"raw_metadata":null,"created_at":"2026-07-19T02:59:32.191237Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}