{"doi":"10.61797/ijbca.v5i2.614","title":"Long-term Toxicity Assessment of Copper Oxide Nanoparticles in Wistar Rats","abstract":"While copper oxide nanoparticles (CuO-NPs) have extensive applications in many sectors, their widespread use has increased the potential for environmental toxicity. This study aimed to determine the hepatic injury and oxidative stress induced by CuO nanoparticles (NPs) in male Wistar rats. They were orally administered CuO-NPs at concentrations of 50, 100 and 200 mg/Kg body weight (bwt), once daily for 28 days. A control group received ultrapure water. Twenty-four hours after completion of the experiment, samples were collected following established procedures. We analyzed several biomarkers and performed a histopathology assessment to evaluate liver damage. Our evaluation included measurements of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) in serum samples, alongside an assessment of reduced glutathione (GSH) levels and microscopic examination of liver tissue morphology. The results of the study indicated that treated rats experienced increased levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH), while experiencing a significant decrease in reduced glutathione (GSH), compared to the control group. Furthermore, histopathological examination of the liver in the treated rats revealed notable morphological alterations compared to the control group. Results from this investigation support the hypothesis that CuO-NP exert hepatotoxicity through the induction of oxidative stress.","journal":"International Journal of Biomedical and Clinical Analysis","year":2025,"id":587027,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9565,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1502546,"name":"P Madhusudhana Chary","orcid":null,"position":1,"is_corresponding":false},{"id":1502547,"name":"S Anitha Kumari","orcid":null,"position":2,"is_corresponding":false},{"id":1323751,"name":"Anita K. Patlolla","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:59:36.020030Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}