{"doi":"10.5754/hge12881","title":"Unknown","abstract":null,"journal":null,"year":null,"id":624273,"datarank":0.6010999777848708,"base_score":4.007333185232471,"endowment":4.007333185232471,"self_citation_contribution":0.6010999777848708,"citation_network_contribution":0.0,"self_endowment_contribution":0.6010999777848708,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":54,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Identification of CD44+ cancer stem cells in human gastric cancer.","abstract":"The cancer stem cell hypothesis suggests that tumors are initiated and maintained by cancer stem cells capable of self-renewal and differentiation into mature tumor cells. Recent studies have demonstrated the existence of cancer initiating cells in different solid tumors. In this study, we identified a subpopulation of CD44+ cells within the tumor of gastric cancer patients, which, upon treatment by chemotherapeutic agent 5-fluorouracil (5-FU), were markedly enriched. In vitro culture of isolated CD44+ subpopulation from gastric tumors by magnetic beads sorting led to formation of gastric spheroid colonies. These colonies retained CD44+ surface marker expression during culture, and were undifferentiated in nature. Subcutaneous injections of CD44+ gastric cancer cells conferred tumorigenicity in SCID mice. Moreover, implantation of CD44+ cells from these established tumors remained tumorigenic in successive passages. Using CD44+ cells isolated from the gastric cell lines AGS and SGC7901, similar results were obtained. Upon enrichment by 5-FU, CD44+ cells harbored increased ALDH expression as compared with CD44- cells. Our results demonstrated for the first time the existence of CD44+ cells within the tumors of gastric cancer patients that are endowed with stem cells properties, and also provide a plausible explanation for chemo-resistance frequently observed in gastric cancer patients. Such findings provide a basis for further studies on targeting this tumorigenic subpopulation for better treatment of gastric cancer patients.","is_dataset_classified":null,"base_score":4.007333185232471,"endowment":4.007333185232471,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23478146","pmcid":null,"openalex_id":"https://openalex.org/W170960211","authors":[],"funders":[],"total_grants":0,"fwci":3.1372,"citation_percentile":0.92026139,"influential_citations":0,"citation_trend":[{"year":2013,"count":4},{"year":2014,"count":4},{"year":2015,"count":5},{"year":2016,"count":7},{"year":2017,"count":8},{"year":2018,"count":5},{"year":2019,"count":3},{"year":2020,"count":6},{"year":2021,"count":3},{"year":2022,"count":4},{"year":2024,"count":3},{"year":2025,"count":2}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://pubmed.ncbi.nlm.nih.gov/23478146","host_type":"repository"},{"url":"http://hdl.handle.net/10722/183839","host_type":"repository"}],"fields_of_study":["Cancer Cells and Metastasis","Animals","Antimetabolites, Antineoplastic","Cell Line, Tumor","Flow Cytometry","Fluorouracil","Humans","Hyaluronan Receptors","Mice","Mice, SCID","Neoplastic Stem Cells","Reverse Transcriptase Polymerase Chain Reaction","Stomach Neoplasms","Tetrazolium Salts","Thiazoles"],"mesh_terms":["Animals","Antimetabolites, Antineoplastic","Flow Cytometry","Fluorouracil","Humans","Stomach Neoplasms","Tetrazolium Salts","Thiazoles","Neoplastic Stem Cells","Mice, SCID","Hyaluronan Receptors","Reverse Transcriptase Polymerase Chain Reaction","Cell Line, Tumor","Mice"],"keywords":["CD44","Cancer stem cell","Cancer","Cancer research","Cancer cell","Stem cell","Biology","Stem cell marker","Pathology","Cell","Medicine","Cell biology","Biochemistry","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-04T03:20:03.393236Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}