{"doi":"10.5588/ijtld.21.0741","title":"Quantification of multidrug-resistant <i>M. tuberculosis</i> bacilli in sputum during the first 8 weeks of treatment","abstract":"SETTING: Mulago Hospital, Kampala, Uganda. OBJECTIVE: To quantify Mycobacterium tuberculosis in sputum during the first 8 weeks of pulmonary multidrug-resistant TB (MDR-TB) treatment. DESIGN: We enrolled consecutive adults with pulmonary MDR-TB treated according to national guidelines. We collected overnight sputum samples before treatment and weekly. Sputum samples were cultured on Middlebrook 7H11S agar to measure colony-forming units per mL (cfu/mL) and in MGIT™ 960™ media to measure time to detection (TTD). Linear mixed-effects regression was used to estimate the relational change in log 10 cfu/mL and TTD. RESULTS: Twelve adults (median age: 27 years) were enrolled. Half were women, and two-thirds were HIV-positive. At baseline, median log 10 cfu/mL was 5.1, decreasing by 0.29 log 10 cfu/mL/week. The median TTD was 116.5 h, increasing in TTD by 36.97 h/week. The weekly change was greater in the first 2 weeks (–1.04 log 10 cfu/mL/week and 120.02 h/week) than in the remaining 6 weeks (–0.17 log 10 cfu/mL/week and 26.11 h/week). CONCLUSION: Serial quantitative culture measures indicate a slow, uneven rate of decline in sputum M. tuberculosis over 8 weeks of standardized pulmonary MDR-TB treatment.","journal":"The International Journal of Tuberculosis and Lung Disease","year":2022,"id":292171,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8891,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":759909,"name":"Kathleen D. Eisenach","orcid":"0000-0002-5426-0970","position":1,"is_corresponding":false},{"id":231145,"name":"Moses Joloba","orcid":"0000-0002-0334-9983","position":2,"is_corresponding":false},{"id":406179,"name":"Willy Ssengooba","orcid":"0000-0002-1643-110X","position":3,"is_corresponding":false},{"id":976347,"name":"Carolyn Namaganda","orcid":null,"position":4,"is_corresponding":false},{"id":253371,"name":"Mary Nsereko","orcid":null,"position":5,"is_corresponding":false},{"id":976348,"name":"Brenda Okware","orcid":null,"position":6,"is_corresponding":false},{"id":951277,"name":"Joseph S. Cavanaugh","orcid":"0000-0003-1866-3841","position":7,"is_corresponding":false},{"id":336080,"name":"J. Peter Cegielski","orcid":"0000-0003-2647-4354","position":8,"is_corresponding":false},{"id":797882,"name":"Sarah E. Smith-Jeffcoat","orcid":"0000-0002-7962-9017","position":0,"is_corresponding":true}],"reference_count":20,"raw_metadata":null,"created_at":"2026-07-19T00:30:42.221508Z","pmid":"36281051","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}