{"doi":"10.54645/202417suppmf-87","title":"Overexpressed yeast Bax inhibitor (Bxi1p/Ybh3p) is a calcium channel in E. coli","abstract":"Human Bax Inhibitor-1 (HsBI-1/TMBIM6) is the founding member of the evolutionary conserved TMBIM superfamily of proteins that share sequence homology within the transmembrane Bax inhibitor-containing motif (TMBIM). Mechanistically, BI-1/TMBIM6 and all the other mammalian TMBIM proteins appear to be involved in the maintenance of calcium homeostasis, and the crystal structure of a bacterial TMBIM protein, BsYetJ, suggests that the protein is a pH-sensitive calcium leak. The budding yeast, Saccharomyces cerevisiae, has a single TMBIM family member (YNL305C) named Bxi1p/Ybh3p. To determine the function Bxi1p/Ybh3p, we overexpressed Bxi1p-GFP in E. coli to interrogate its putative calcium channel function. We show that bacterial cells expressing Bxi1p-GFP are more permeable to calcium than controls. Our data suggests that yeast Bax inhibitor (Bxi1p) is a calcium channel in E. coli, lending support to our proposal that Bxi1p is a bona fide member of the TMBIM family of proteins. Finally, parallel experiments also revealed that the human Bax Inhibitor-1 (HsBI1/TMBIM6) is also a calcium channel in bacteria.","journal":"SciEnggJ","year":2024,"id":495019,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9504,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1002650,"name":"John Kalhorn","orcid":null,"position":1,"is_corresponding":false},{"id":1002648,"name":"Julia Aguiar","orcid":null,"position":2,"is_corresponding":false},{"id":1002649,"name":"Madelyn Crago","orcid":null,"position":3,"is_corresponding":false},{"id":1002369,"name":"Nicholas Mello","orcid":"0000-0002-7310-747X","position":4,"is_corresponding":false},{"id":1002651,"name":"Amanda Raffa","orcid":null,"position":5,"is_corresponding":false},{"id":1341825,"name":"Alexander Strakosha","orcid":null,"position":6,"is_corresponding":false},{"id":536734,"name":"Nicanor Pier Giorgio Austriaco","orcid":"0000-0003-1854-8181","position":7,"is_corresponding":false},{"id":327198,"name":"James M. Mullin","orcid":"0000-0001-5285-7530","position":0,"is_corresponding":true}],"reference_count":35,"raw_metadata":null,"created_at":"2026-07-19T02:09:15.528853Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}