{"doi":"10.52843/cassyni.kkmwn6","title":"Introduction to the 6th Biometals Webinars, Mechanistic studies of the bacterial nitrosative stress sensor NsrR","abstract":"Mechanistic studies of the bacterial nitrosative stress sensor NsrR Nitric oxide (NO) is a ubiquitous signalling molecule and cytotoxic agent. In order to survive and thrive, organisms must be able to respond to NO, and the regulatory protein NsrR, a member of the Rrf2 protein superfamily, plays a major role in this as it is found in a wide range of benign and pathogenic bacteria. NsrR binds a [4Fe-4S] cluster as a cofactor and, in the absence of NO, represses transcription of genes primarily involved NO detoxification. Previously, we have shown that [4Fe-4S] NsrR reacts rapidly with NO, in a complex reaction involving multiple NO molecules per cluster, resulting in formation of NsrR-bound iron-nitrosyl species [3]. Here, we sought to investigate the reaction of [4Fe-4S] NsrR bound to DNA, in order to determine when high affinity DNA-binding is lost. Using surface plasmon resonance (SPR) and native mass spectrometry (MS) NsrR binding to the promoter regions of two NsrR-regulated genes, hmpA1 and hmpA2 was probed, revealing the nature of binding and the reaction of DNA-bound NsrR with NO in remarkable detail.","journal":null,"year":2024,"id":500515,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9611,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1349812,"name":"Nick E. Le Brun","orcid":"0000-0001-9780-4061","position":1,"is_corresponding":false},{"id":1339745,"name":"Isabelle Michaud‐Soret","orcid":"0000-0002-8936-4964","position":0,"is_corresponding":true}],"reference_count":3,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:10:08.435215Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}