{"doi":"10.52601/bpr.2025.250041","title":"Allosteric regulation of C-reactive protein","abstract":"Serum-soluble C-reactive protein (CRP) is a highly conserved innate immune pattern recognition molecule comprised of five identical globular subunits arranged in a cyclic pentameric shape. While the interactions between each of the five subunits are noncovalent, each monomer possesses an intra-subunit disulfide bond linking adjacent β-strands within the same β-sheet (ie, a cross-strand disulfide bond). This bond is necessary for the folding and polymerization of CRP during its synthesis, but also serves as a regulator of CRP function. Reduction of the bond initiates a conformational, allosteric change that exposes a hidden binding site for cholesterol and amplifies CRP-mediated cellular responses. In this review, we discuss the folding, multimerization, and structural changes CRP undergoes during its life cycle. We also highlight the role of the cross-strand disulfide bond in CRP, both for its contributions to the assembly of the pentamer and for its role as an allosteric regulator of CRP function.","journal":"Biophysics Reports","year":2025,"id":575880,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.954,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1026854,"name":"Ibraheem M. Rajab","orcid":"0000-0003-3751-0461","position":1,"is_corresponding":false},{"id":753422,"name":"Margaret E. Olson","orcid":"0000-0003-1865-9615","position":2,"is_corresponding":false},{"id":1484451,"name":"Jianmin Lv","orcid":null,"position":3,"is_corresponding":false},{"id":355418,"name":"Zhenyu Yao","orcid":"0000-0002-1144-0621","position":4,"is_corresponding":false},{"id":1026855,"name":"Lawrence A. Potempa","orcid":"0000-0002-9267-1041","position":5,"is_corresponding":false},{"id":602353,"name":"Marc Potempa","orcid":"0000-0003-3853-3948","position":0,"is_corresponding":true}],"reference_count":93,"raw_metadata":null,"created_at":"2026-07-19T02:57:52.712371Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}