{"doi":"10.51253/pafmj.v72i1.7393","title":"Effect of obestatin on pituitary gonadal axis, leptin and MDA levels in type 2 diabetic Sprague Dawley Rats","abstract":"Objective: To demonstrate the effect of Obestatin supplementation on oxidative stress and serum levels of testosterone, follicular stimulating hormone, luteinizing hormone and leptin in type 2 diabetic rats.
 Study Design: Laboratory-based experimental study
 Place and Duration of Study: Physiology Department, Army Medical College Rawalpindi from Mar-June 2015.
 Methodology: Forty-five healthy male Sprague Dawley rats were randomly divided into three groups. Group-I (healthy rats) were fed with regular pellet diet (NPD). Group-II (diabetic rats) and group-III (Obestatin treated diabetic rats) were fed with high-fat diet (HFD) followed by a single IP injection of Streptozotocin in the dose of 35 mg/kg on 15th day. After ten weeks, group-III was treated with intraperitoneal Obestatin (1 nmol/100ml). Blood samples were obtained by terminal intra-cardiac sampling for bioassays of Insulin, testosterone, FSH, LH, MDA and leptin by ELISA.
 Results: Obestatin supplementation in diabetic rats resulted in significant increase in FSH (9.4 ± 0.74 ng/dL), LH (3.89 ± 0.10 ng/dL) and testosterone levels (2.01 ± 0.09 ng/dL) in comparison with non-treated diabetic rats (7.04 ± 0.50 ng/dL), (1.70 ± 0.28 ng/dL) and (1.10 ± 0.40 ng/dL) respectively. Whereas leptin (3.91 ± 0.24 ng/dL) and MDA levels (1.75 ± 0.22 ng/dL) were reduced significantly when compared to the healthy rats (6.10 ± 1.29 ng/dL) and (1.96 ± 0.07 ng/dL) respectively.
 Conclusion: Testosterone levels are increased by Obestatin supplementation in type 2 diabetic rats by stimulating the pituitary-gonadal axis due to a decrease in circulating leptin levels and oxidative stress.","journal":"Pakistan Armed Forces Medical Journal","year":2022,"id":314031,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.8827,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1012957,"name":"Amina Rasul","orcid":null,"position":1,"is_corresponding":false},{"id":1012649,"name":"Sajid Ali","orcid":"0000-0002-1477-6876","position":2,"is_corresponding":false},{"id":1012958,"name":"Fauzia Mazloum","orcid":null,"position":3,"is_corresponding":false},{"id":1012959,"name":"Shoaib Bin Aleem","orcid":null,"position":4,"is_corresponding":false},{"id":1012960,"name":"Amber Farooq","orcid":null,"position":5,"is_corresponding":false},{"id":1012956,"name":"Nadia Latif","orcid":null,"position":0,"is_corresponding":true}],"reference_count":21,"raw_metadata":null,"created_at":"2026-07-19T00:33:48.490749Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}