{"doi":"10.5114/bta.2023.132775","title":"In vitro immune evaluation of adenoviralvector-based platform for infectious diseases","abstract":"New prophylactic vaccine platforms are imperative to combat respiratory infections. The efficacy of T and B memory cell-mediated protection, generated through the adenoviral vector, was tested to assess the effectiveness of the new adenoviral-based platforms for infectious diseases. A combination of adenovirus AdV1 (adjuvant), armed with costimulatory ligands (ICOSL and CD40L), and rRBD (antigen: recombinant nonglycosylated spike protein rRBD) was used to promote the differentiation of T and B lymphocytes. Adenovirus AdV2 (adjuvant), without ligands, in combination with rRBD, served as a control. <i>In vitro</i> T-cell responses to the AdV1+rRBD combination revealed that CD8+ platform-specific T-cells increased (37.2±0.7% vs. 23.1±2.1%), and T-cells acted against SARS-CoV-2 via CD8+TEMRA (50.0±1.3% vs. 36.0±3.2%). Memory B cells were induced after treatment with either AdV1+rRBD (84.1±0.8% vs. 82.3±0.4%) or rRBD (94.6±0.3% vs. 82.3±0.4%). Class-switching from IgM and IgD to isotype IgG following induction with rRBD+Ab was observed. RNA-seq profiling identified gene expression patterns related to T helper cell differentiation that protect against pathogens. The analysis determined signaling pathways controlling the induction of protective immunity, including the MAPK cascade, adipocytokine, cAMP, TNF, and Toll-like receptor signaling pathway. The AdV1+rRBD formulation induced IL-6, IL-8, and TNF. RNA-seq of the VERO E6 cell line showed differences in the apoptosis gene expression stimulated with the platforms vs. mock. In conclusion, AdV1+rRBD effectively generates T and B memory cell-mediated protection, presenting promising results in producing CD8+ platform-specific T cells and isotype-switched IgG memory B cells. The platform induces protective immunity by controlling the Th1, Th2, and Th17 cell differentiation gene expression patterns. Further studies are required to confirm its effectiveness.","journal":"BioTechnologia","year":2023,"id":382905,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9593,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":751905,"name":"Łukasz Kuryk","orcid":"0000-0003-1887-6361","position":1,"is_corresponding":false},{"id":963305,"name":"Teresa Szczepińska","orcid":"0000-0001-9749-1253","position":2,"is_corresponding":false},{"id":1113712,"name":"Michał Łaźniewski","orcid":"0000-0003-0436-404X","position":3,"is_corresponding":false},{"id":857845,"name":"Mariangela Garofalo","orcid":"0000-0002-5001-7826","position":4,"is_corresponding":false},{"id":1029446,"name":"Anna Mazurkiewicz-Pisarek","orcid":"0000-0001-9097-8894","position":5,"is_corresponding":false},{"id":1149405,"name":"Diana Mikiewicz","orcid":"0000-0002-0954-291X","position":6,"is_corresponding":false},{"id":1149786,"name":"Alina Mazurkiewicz","orcid":null,"position":7,"is_corresponding":false},{"id":1149406,"name":"Maciej Trzaskowski","orcid":"0000-0001-5892-1898","position":8,"is_corresponding":false},{"id":895338,"name":"Magdalena Wieczorek","orcid":"0000-0001-8770-5131","position":9,"is_corresponding":false},{"id":895337,"name":"Katarzyna Pancer","orcid":"0000-0003-3665-5765","position":10,"is_corresponding":false},{"id":541208,"name":"Ewelina Hallmann","orcid":"0000-0001-9205-1910","position":11,"is_corresponding":false},{"id":430972,"name":"Lidia B. 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