{"doi":"10.4269/ajtmh.22-0715","title":"Plasmodium falciparum with pfhrp2/3 Deletion Not Detected in a 2018–2021 Malaria Longitudinal Cohort Study in Kinshasa Province, Democratic Republic of the Congo","abstract":"Histidine-rich protein 2- (HRP2-) based rapid diagnostic tests (RDTs) are widely used to detect Plasmodium falciparum in sub-Saharan Africa. Reports of parasites with pfhrp2 and/or pfhrp3 (pfhrp2/3) gene deletions in Africa raise concerns about the long-term viability of HRP2-based RDTs. We evaluated changes in pfhrp2/3 deletion prevalence over time using a 2018-2021 longitudinal study of 1,635 enrolled individuals in Kinshasa Province, Democratic Republic of the Congo (DRC). Samples collected during biannual household visits with ≥ 100 parasites/µL by quantitative real-time polymerase chain reaction were genotyped using a multiplex real-time PCR assay. Among 2,726 P. falciparum PCR-positive samples collected from 993 participants during the study period, 1,267 (46.5%) were genotyped. No pfhrp2/3 deletions or mixed pfhrp2/3-intact and -deleted infections were identified in our study. Pfhrp2/3-deleted parasites were not detected in Kinshasa Province; ongoing use of HRP2-based RDTs is appropriate.","journal":"American Journal of Tropical Medicine and Hygiene","year":2023,"id":377097,"datarank":0.20794415416798362,"base_score":1.3862943611198906,"endowment":1.3862943611198906,"self_citation_contribution":0.20794415416798362,"citation_network_contribution":0.0,"self_endowment_contribution":0.20794415416798362,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9586,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":487448,"name":"Melchior Mwandagalirwa Kashamuka","orcid":null,"position":1,"is_corresponding":false},{"id":773909,"name":"Kristin Banek","orcid":"0000-0001-9219-2852","position":2,"is_corresponding":false},{"id":717271,"name":"Joseph A. Bala","orcid":null,"position":3,"is_corresponding":false},{"id":717272,"name":"Marthe Nkalani","orcid":null,"position":4,"is_corresponding":false},{"id":717273,"name":"Georges Kihuma","orcid":null,"position":5,"is_corresponding":false},{"id":595322,"name":"Joseph Atibu","orcid":null,"position":6,"is_corresponding":false},{"id":1011908,"name":"Georges Emo Mahilu","orcid":null,"position":7,"is_corresponding":false},{"id":272757,"name":"Kyaw L. Thwai","orcid":null,"position":8,"is_corresponding":false},{"id":785596,"name":"Ashenafi Assefa","orcid":"0000-0001-6093-6685","position":9,"is_corresponding":false},{"id":242456,"name":"Jeffrey A. Bailey","orcid":"0000-0002-6899-8204","position":10,"is_corresponding":false},{"id":382460,"name":"Rhoel R. Dinglasan","orcid":"0000-0001-5433-8179","position":11,"is_corresponding":false},{"id":270632,"name":"Jonathan J. Juliano","orcid":"0000-0002-0591-0850","position":12,"is_corresponding":false},{"id":270631,"name":"Antoinette Tshefu","orcid":"0000-0001-7657-6901","position":13,"is_corresponding":false},{"id":270623,"name":"Jonathan B. Parr","orcid":"0000-0002-4465-9830","position":14,"is_corresponding":false},{"id":955784,"name":"Ruthly François","orcid":"0000-0001-7807-5799","position":0,"is_corresponding":true}],"reference_count":33,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:16:36.370283Z","pmid":"37339759","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}