{"doi":"10.4269/ajtmh.1999.60.693","title":"Rapid serologic diagnosis of dengue virus infection using a commercial capture ELISA that distinguishes primary and secondary infections.","abstract":"<jats:p>A commercial capture ELISA for specific IgM and IgG antibodies produced during dengue infection (PanBio Dengue Duo) showed excellent sensitivity (99%, n = 78) using sera collected at hospital discharge compared with established ELISA and hemagglutination inhibition (HAI) assays. Furthermore, the ELISA was able to diagnose 79% of the dengue cases using sera collected at hospital admission. The ELISA also showed high specificity (92%) in paired sera from patients without flavivirus infection (n = 26), although 45% of the patients with Japanese encephalitis (n = 20) showed elevation of IgG but not IgM. The IgG capture ELISA showed good correlation with the HAI assay (r = 0.83, P &lt; 0.0001), and IgG levels could be used to distinguish between primary and secondary infection, with 100% of primary infections and 96% of secondary infections being correctly classified. This ELISA should prove useful in the clinical diagnosis of dengue infections.</jats:p>","journal":"The American journal of tropical medicine and hygiene","year":1999,"id":605525,"datarank":0.7324202883879557,"base_score":4.882801922586371,"endowment":4.882801922586371,"self_citation_contribution":0.7324202883879557,"citation_network_contribution":0.0,"self_endowment_contribution":0.7324202883879557,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":131,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1325314,"name":"A Nisalak","orcid":null,"position":1,"is_corresponding":false},{"id":1554068,"name":"T Solomon","orcid":null,"position":2,"is_corresponding":false},{"id":1554069,"name":"S Kalayanarooj","orcid":null,"position":3,"is_corresponding":false},{"id":1554070,"name":"M D Nguyen","orcid":null,"position":4,"is_corresponding":false},{"id":1554071,"name":"R Kneen","orcid":null,"position":5,"is_corresponding":false},{"id":1554072,"name":"A Cuzzubbo","orcid":null,"position":6,"is_corresponding":false},{"id":1554073,"name":"P L Devine","orcid":null,"position":7,"is_corresponding":false},{"id":1554067,"name":"D W Vaughn","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Rapid serologic diagnosis of dengue virus infection using a commercial capture ELISA that distinguishes primary and secondary infections.","abstract":"<jats:p>A commercial capture ELISA for specific IgM and IgG antibodies produced during dengue infection (PanBio Dengue Duo) showed excellent sensitivity (99%, n = 78) using sera collected at hospital discharge compared with established ELISA and hemagglutination inhibition (HAI) assays. Furthermore, the ELISA was able to diagnose 79% of the dengue cases using sera collected at hospital admission. The ELISA also showed high specificity (92%) in paired sera from patients without flavivirus infection (n = 26), although 45% of the patients with Japanese encephalitis (n = 20) showed elevation of IgG but not IgM. The IgG capture ELISA showed good correlation with the HAI assay (r = 0.83, P &lt; 0.0001), and IgG levels could be used to distinguish between primary and secondary infection, with 100% of primary infections and 96% of secondary infections being correctly classified. 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