{"doi":"10.4269/ajtmh.14-0236","title":"Deficiency in Mannose-Binding Lectin-Associated Serine Protease-2 Does Not Increase Susceptibility to Trypanosoma cruzi Infection","abstract":"<jats:p><jats:italic>Trypanosoma cruzi</jats:italic> is the causative agent of Chagas' disease, a chronic illness affecting 10 million people around the world. The complement system plays an important role in fighting microbial infections. The recognition molecules of the lectin pathway of complement activation, mannose-binding lectin (MBL), ficolins, and CL-11, bind to specific carbohydrates on pathogens, triggering complement activation through MBL-associated serine protease-2 (MASP-2). Previous <jats:italic>in vitro</jats:italic> work showed that human MBL and ficolins contribute to <jats:italic>T. cruzi</jats:italic> lysis. However, MBL-deficient mice are only moderately compromised in their defense against the parasite, as they may still activate the lectin pathway through ficolins and CL-11. Here, we assessed MASP-2-deficient mice, the only presently available mouse line with total lectin pathway deficiency, for a phenotype in <jats:italic>T. cruzi</jats:italic> infection. Total absence of lectin pathway functional activity did not confer higher susceptibility to <jats:italic>T. cruzi</jats:italic> infection, suggesting that it plays a minor role in the immune response against this parasite.</jats:p>","journal":"The American Society of Tropical Medicine and Hygiene","year":2015,"id":17742,"datarank":0.5420770986770038,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.16934110120880375,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.16934110120880375,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":9,"citers_with_citation_signal":8,"citers_with_endowment":8,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":125852,"name":"Nicholas J. Lynch","orcid":null,"position":1,"is_corresponding":false},{"id":125853,"name":"Cordula M. Stover","orcid":null,"position":2,"is_corresponding":false},{"id":125854,"name":"Youssif M. Ali","orcid":null,"position":3,"is_corresponding":false},{"id":125855,"name":"Carolina Valck","orcid":null,"position":4,"is_corresponding":false},{"id":125856,"name":"Francisca Noya-Leal","orcid":null,"position":5,"is_corresponding":false},{"id":124944,"name":"Wilhelm J. Schwaeble","orcid":null,"position":6,"is_corresponding":false},{"id":125857,"name":"Arturo Ferreira","orcid":null,"position":7,"is_corresponding":false},{"id":125851,"name":"Carolina H. Ribeiro","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"25548381","pmcid":"PMC4347335","openalex_id":"https://openalex.org/W2328134015","authors":[],"funders":[{"funder_name":"Medical Research Council","grant_id":"G0801952","title":null}],"total_grants":1,"fwci":0.8626,"citation_percentile":0.76136716,"influential_citations":0,"citation_trend":[{"year":2015,"count":1},{"year":2016,"count":2},{"year":2017,"count":1},{"year":2018,"count":1},{"year":2019,"count":2},{"year":2021,"count":3},{"year":2025,"count":1}],"oa_status":"bronze","license":"cc-by-nc-nd","oa_locations":[{"url":"http://www.ajtmh.org/deliver/fulltext/14761645/92/2/320.pdf?itemId=%2Fcontent%2Fjournals%2F10.4269%2Fajtmh.14-0236&mimeType=pdf&containerItemId=content/journals/14761645","host_type":"journal"},{"url":"http://www.ajtmh.org/deliver/fulltext/14761645/92/2/320.pdf?itemId=%2Fcontent%2Fjournals%2F10.4269%2Fajtmh.14-0236&mimeType=pdf&containerItemId=content/journals/14761645","host_type":"BRONZE"},{"url":"http://www.ajtmh.org/deliver/fulltext/14761645/92/2/320.pdf?itemId=%2Fcontent%2Fjournals%2F10.4269%2Fajtmh.14-0236&mimeType=pdf&containerItemId=content/journals/14761645","host_type":"publisher"},{"url":"https://www.ajtmh.org/view/journals/tpmd/92/2/article-p320.xml","host_type":"publisher"},{"url":"https://www.ajtmh.org/downloadpdf/journals/tpmd/92/2/article-p320.xml","host_type":"publisher"},{"url":"https://doi.org/10.4269/ajtmh.14-0236","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/25548381","host_type":"repository"},{"url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.988.781","host_type":""},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4347335","host_type":"repository"},{"url":"https://repositorio.uchile.cl/handle/2250/166353","host_type":"repository"},{"url":"https://repositorio.uchile.cl/handle/2250/166458","host_type":"repository"}],"fields_of_study":["Trypanosoma species research and implications","Complement system in diseases","Research on Leishmaniasis Studies","Medicine","Biology","Animals","Chagas Disease","Complement Activation","Disease Susceptibility","Female","Humans","Male","Mannose-Binding Protein-Associated Serine Proteases","Mice","Mice, Inbred C57BL","Parasite Load","Trypanosoma cruzi"],"mesh_terms":["Animals","Complement Activation","Disease Susceptibility","Female","Humans","Male","Mice, Inbred C57BL","Trypanosoma cruzi","Chagas Disease","Mannose-Binding Protein-Associated Serine Proteases","Mice","Parasite Load"],"keywords":["Mannan-binding lectin","Lectin pathway","Ficolin","Complement system","Biology","Lectin","Trypanosoma cruzi","MASP1","C-type lectin","Proteases","Microbiology","Chagas disease","Collectin","Serine protease","Immunology","Protease","Alternative complement pathway","Immune system","Innate immune system","Parasite hosting","Biochemistry","Enzyme"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-06-02T21:21:22.509362Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}