{"doi":"10.4247/am.2015.abf117","title":"The Role and Mechanism of VCAM-1 in 2-Methoxyestradiol Inhibiting Monocyte-Endothelial Cell Adhesions","abstract":null,"journal":"Adaptive Medicine","year":2015,"id":669029,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":804100,"name":"Chen Wang","orcid":"0009-0005-6441-5939","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"The Role and Mechanism of VCAM-1 in 2-Methoxyestradiol Inhibiting Monocyte-Endothelial Cell Adhesions","abstract":"2-Methoxyestradiol (2-ME) is a major endogenous metabolite of estrogen, which formed by catalysis of cytochrome P450 (CYP450) and catechins-O-methoxy transferase (COMT), has an important role in atherosclerosis (AS), but the underlying mechanisms remain obscure. In our work, we investigated the role and mechanism of 2-ME inhibiting monocyte-endothelial cell adhesion. In vitro studies using monocyte-endothelial cell adhesion assay and cell immunofluorescence method showed that lipopolysaccharide (LPS) promoted monocyte-endothelial cell adhesion, these effects were blocked by 2-ME through a dose- and time-dependent manner. Furthermore, Vascular cell adhesion molecular-1 (VCAM-1) specific antibody (anti- VCAM-1, 1:1000) were pre-incubated the cells for 48 h, and by monocyte-endothelial cell adhesion experiment, results showed that anti-VCAM-1 can significantly inhibit the LPS-induced monocyte-endothelial cell adhesion, indicating VCAM-1 may be the key molecule of the LPS-nduced cell adhesion. Moreover, through Western Blot and monocyte-endothelial cell adhesion assay observing, results showed that 2-ME (10^(-5) M) pretreatment for 48 h was strongest inhibited the expression of VCAM-1, NF-_κBp65 nucleus protein and nuclear translocation on LPS-induced endothelial cell. In conclusion, 2-ME has the effect on monocytes-endothelial cell adhesion, and the mechanism is that inhibiting the role of the nuclear transcription factor NF-_κBp65 and down regulation the expression of VCAM-1. This study will be helpful for further clarify the mechanism of 2-ME anti-AS, and can be used as a new type of clinical drug development.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"19162232","pmcid":null,"openalex_id":"https://openalex.org/W2519820356","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.22342368,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://doi.org/10.4247/am.2015.abf117","host_type":"journal"}],"fields_of_study":["Cell Adhesion Molecules Research","Estrogen and related hormone effects","Atherosclerosis and Cardiovascular Diseases"],"mesh_terms":[],"keywords":["VCAM-1","Cell adhesion molecule","Cell adhesion","Endothelial stem cell","Monocyte","Chemistry","Cell biology","Adhesion","Soluble cell adhesion molecules","Cell","Biology","Biochemistry","Immunology","In vitro"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-14T13:37:01.614977Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}