{"doi":"10.4161/cc.26988","title":"Dynamics of histone H3.3 deposition in proliferating and senescent cells reveals a DAXX-dependent targeting to PML-NBs important for pericentromeric heterochromatin organization","abstract":null,"journal":"Cell Cycle","year":2014,"id":621312,"datarank":0.6476232170304466,"base_score":4.31748811353631,"endowment":4.31748811353631,"self_citation_contribution":0.6476232170304466,"citation_network_contribution":0.0,"self_endowment_contribution":0.6476232170304466,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":74,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":6,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":293810,"name":"Teresa Olbrich","orcid":null,"position":1,"is_corresponding":false},{"id":1604219,"name":"Myriam Gwerder","orcid":null,"position":2,"is_corresponding":false},{"id":1604220,"name":"Daniel Fink","orcid":null,"position":3,"is_corresponding":false},{"id":497823,"name":"Manuel Stucki","orcid":"0000-0002-9974-0134","position":4,"is_corresponding":false},{"id":1604218,"name":"Armelle Corpet","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Dynamics of histone H3.3 deposition in proliferating and senescent cells reveals a DAXX-dependent targeting to PML-NBs important for pericentromeric heterochromatin organization","abstract":"Oncogene-induced senescence is a permanent cell cycle arrest characterized by extensive chromatin reorganization. Here, we investigated the specific targeting and dynamics of histone H3 variants in human primary senescent cells. We show that newly synthesized epitope-tagged H3.3 is incorporated in senescent cells but does not accumulate in senescence-associated heterochromatin foci (SAHF). Instead, we observe that new H3.3 colocalizes with its specific histone chaperones within the promyelocytic leukemia nuclear bodies (PML-NBs) and is targeted to PML-NBs in a DAXX-dependent manner both in proliferating and senescent cells. We further show that overexpression of DAXX enhances targeting of H3.3 in large PML-NBs devoid of transcriptional activity and promotes the accumulation of HP1, independently of H3K9me3. Loss of H3.3 from pericentromeric heterochromatin upon DAXX or PML depletion suggests that the targeting of H3.3 to PML-NBs is implicated in pericentromeric heterochromatin organization. Together, our results underline the importance of the replication-independent chromatin assembly pathway for histone replacement in non-dividing senescent cells and establish PML-NBs as important regulatory sites for the incorporation of new H3.3 into chromatin.","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":6,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"24200965","pmcid":"PMC3906242","openalex_id":null,"authors":[],"funders":[{"funder_name":"Swiss National Science Foundation","grant_id":"144284","title":"MDC1 and NBS1: cellular functions and human disease"},{"funder_name":"Swiss National Science Foundation","grant_id":"139706","title":"IRIF versus SDF: role of the ?H2A.X chromatin domain in oncogene-induced cellular senescence"}],"total_grants":2,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":"hybrid","license":"cc-by-nc","oa_locations":[{"url":"https://www.tandfonline.com/doi/pdf/10.4161/cc.26988?needAccess=true","host_type":"publisher"},{"url":"http://www.tandfonline.com/doi/pdf/10.4161/cc.26988","host_type":"publisher"},{"url":"https://doi.org/10.5167/uzh-88292","host_type":""},{"url":"https://europepmc.org/articles/PMC3906242","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3906242?pdf=render","host_type":"Europe_PMC"},{"url":"https://doi.org/10.4161/cc.26988","host_type":""},{"url":"https://dx.doi.org/10.5167/uzh-88292","host_type":""},{"url":"https://pubmed.ncbi.nlm.nih.gov/24200965","host_type":""},{"url":"http://dx.doi.org/10.4161/cc.26988","host_type":""},{"url":"https://dx.doi.org/10.4161/cc.26988","host_type":""},{"url":"https://sonar.ch/global/documents/128875","host_type":""},{"url":"https://www.zora.uzh.ch/id/eprint/88292/","host_type":""}],"fields_of_study":["0301 basic medicine","03 medical and health sciences","0303 health sciences"],"mesh_terms":["Cell Line","Cell Nucleus","Euchromatin","Heterochromatin","Humans","Leukemia, Promyelocytic, Acute","DNA Helicases","Adaptor Proteins, Signal Transducing","Molecular Chaperones","Nuclear Proteins","Histones","DNA, Satellite","Cell Proliferation","Chromatin Assembly and Disassembly","Co-Repressor Proteins","Primary Cell Culture","X-linked Nuclear Protein","Cellular Senescence"],"keywords":["Senescence","Chromatin Dynamics","Daxx","Pml-nbs","H3.3","X-linked Nuclear Protein","Primary Cell Culture","610 Medicine & health","DNA, Satellite","Cell Line","1309 Developmental Biology","1307 Cell Biology","Euchromatin","Histones","Leukemia, Promyelocytic, Acute","Report","Heterochromatin","1312 Molecular Biology","Humans","610 Medicine & health","Cellular Senescence","Adaptor Proteins, Signal Transducing","Cell Proliferation","Cell Nucleus","DNA Helicases","Nuclear Proteins","Chromatin Assembly and Disassembly","10174 Clinic for Gynecology","Co-Repressor Proteins","Molecular Chaperones"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.24779207.v1","title":"Additional file 1 of Pediatric glioma histone H3.3 K27M/G34R mutations drive abnormalities in PML nuclear bodies","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.24779207","title":"Additional file 1 of Pediatric glioma histone H3.3 K27M/G34R mutations drive abnormalities in PML nuclear bodies","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.24779363.v1","title":"Additional file 2 of Pediatric glioma histone H3.3 K27M/G34R mutations drive abnormalities in PML nuclear bodies","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.24779363","title":"Additional file 2 of Pediatric glioma histone H3.3 K27M/G34R mutations drive abnormalities in PML nuclear bodies","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.26651274.v1","title":"Additional file 3 of Pediatric glioma histone H3.3 K27M/G34R mutations drive abnormalities in PML nuclear bodies","publisher":"figshare","resource_type":"JournalArticle"},{"doi":"10.6084/m9.figshare.26651274","title":"Additional file 3 of Pediatric glioma histone H3.3 K27M/G34R mutations drive abnormalities in PML nuclear bodies","publisher":"figshare","resource_type":"JournalArticle"}],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-03T14:01:44.979734Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}