{"doi":"10.4161/cbt.6.5.3960","title":"Caspase-8 preferentially senses the apoptosis-inducing action of NG-18, A gambogic acid derivative, in human leukemia HL-60 cells","abstract":null,"journal":"Cancer Biology & Therapy","year":2007,"id":679410,"datarank":0.5641800173540344,"base_score":3.7612001156935624,"endowment":3.7612001156935624,"self_citation_contribution":0.5641800173540344,"citation_network_contribution":0.0,"self_endowment_contribution":0.5641800173540344,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":42,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1775126,"name":"Yunlong Zhou","orcid":null,"position":1,"is_corresponding":false},{"id":1775127,"name":"Jinjian Lu","orcid":null,"position":2,"is_corresponding":false},{"id":1775128,"name":"Wenhu Duan","orcid":null,"position":3,"is_corresponding":false},{"id":1775130,"name":"Xinxia He","orcid":null,"position":4,"is_corresponding":false},{"id":1775132,"name":"Liping Lin","orcid":null,"position":5,"is_corresponding":false},{"id":1346050,"name":"Jian Ding","orcid":"0000-0001-8788-1418","position":6,"is_corresponding":false},{"id":1669119,"name":"Yuxin Qin","orcid":null,"position":7,"is_corresponding":false},{"id":1775125,"name":"Zhijian Tao","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Caspase-8 preferentially senses the apoptosis-inducing action of NG-18, A gambogic acid derivative, in human leukemia HL-60 cells","abstract":"Gambogic acid (GA) is the major active ingredient of gamboge secreted from a Chinese traditional medicine Garcinia hanburryi possessing potent anti-tumor activity. N-(2-ethoxyethyl)gambogamide (NG-18), a derivative of GA, also efficiently inhibits proliferation of cultured human tumor cells. The inhibition effect of NG-18 is associated with its ability to induce apoptosis. In the present study, NG-18 markedly induced leukemia HL-60 cells apoptosis, and the extrinsic and intrinsic apoptosis pathways were activated almost at the same time. NG-18-induced tumor cell apoptosis was associated with up-regulation of pro-apoptotic Bcl-2 family member Bax, and downregulation of anti-apoptotic protein Bcl-2. The NG-18-induced apoptosis was blocked completely by a pan-caspase inhibitor Z-VAD-FMK, indicating that caspases were functionally and actively involved in this process. The specific inhibition of caspase-8 activity using Z-IETD-FMK significantly blocked NG-18-induced apoptosis. In contrast, inhibition of other initiator caspases, caspase-2 or -9, using Z-VDVAD-FMK or Z-LEHD-FMK respectively had no effect on NG-18-induced apoptosis. Altogether, our data demonstrated that NG-18-induced apoptosis was dependent on caspases and caspase-8 acted as a key executor in the event.","is_dataset_classified":null,"base_score":3.7612001156935624,"endowment":3.7612001156935624,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"17426447","pmcid":null,"openalex_id":"https://openalex.org/W2078800147","authors":[],"funders":[],"total_grants":0,"fwci":5.2328,"citation_percentile":0.94834642,"influential_citations":0,"citation_trend":[{"year":2012,"count":5},{"year":2013,"count":5},{"year":2014,"count":4},{"year":2015,"count":3},{"year":2016,"count":2},{"year":2017,"count":2},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2024,"count":1}],"oa_status":"bronze","license":null,"oa_locations":[{"url":"https://www.tandfonline.com/doi/pdf/10.4161/cbt.6.5.3960?needAccess=true&role=button","host_type":"journal"},{"url":"https://www.tandfonline.com/doi/pdf/10.4161/cbt.6.5.3960?needAccess=true&role=button","host_type":"publisher"},{"url":"http://www.tandfonline.com/doi/pdf/10.4161/cbt.6.5.3960","host_type":"publisher"},{"url":"https://doi.org/10.4161/cbt.6.5.3960","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/17426447","host_type":"repository"}],"fields_of_study":["Natural Compound Pharmacology Studies","Biological Activity of Diterpenoids and Biflavonoids","Synthesis of Organic Compounds","Antineoplastic Agents","Apoptosis","Blotting, Western","Caspase 8","Cell Proliferation","Cytochromes c","Drugs, Chinese Herbal","Flow Cytometry","Garcinia","HL-60 Cells","Humans","Molecular Structure","Poly(ADP-ribose) Polymerases","Proto-Oncogene Proteins c-bcl-2","Xanthones","bcl-X Protein"],"mesh_terms":["Antineoplastic Agents","Drugs, Chinese Herbal","Flow Cytometry","Humans","Poly(ADP-ribose) Polymerases","Blotting, Western","Molecular Structure","Apoptosis","HL-60 Cells","Proto-Oncogene Proteins c-bcl-2","Garcinia","Xanthones","Cytochromes c","Cell Proliferation","bcl-X Protein","Caspase 8"],"keywords":["Gambogic acid","Apoptosis","Caspase","Cell biology","Caspase-9","Chemistry","Intrinsic apoptosis","Programmed cell death","Caspase 8","Biology","Biochemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T13:06:33.222187Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}