{"doi":"10.4161/auto.19652","title":"Dual roles of Atg8−PE deconjugation by Atg4 in autophagy","abstract":null,"journal":"Autophagy","year":2012,"id":601834,"datarank":0.8227195400235984,"base_score":5.484796933490655,"endowment":5.484796933490655,"self_citation_contribution":0.8227195400235984,"citation_network_contribution":0.0,"self_endowment_contribution":0.8227195400235984,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":240,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":245739,"name":"Tao Ni","orcid":"0000-0001-7268-0306","position":1,"is_corresponding":false},{"id":1543249,"name":"Bing Hong","orcid":null,"position":2,"is_corresponding":false},{"id":1543250,"name":"Hai-Yan Wang","orcid":null,"position":3,"is_corresponding":false},{"id":1543251,"name":"Fen-Jun Jiang","orcid":null,"position":4,"is_corresponding":false},{"id":1543252,"name":"Shenshen Zou","orcid":null,"position":5,"is_corresponding":false},{"id":546964,"name":"Yong Chen","orcid":"0000-0002-9820-1614","position":6,"is_corresponding":false},{"id":1515610,"name":"Xi-Long Zheng","orcid":null,"position":7,"is_corresponding":false},{"id":71199,"name":"Daniel J. Klionsky","orcid":"0000-0002-7828-8118","position":8,"is_corresponding":false},{"id":1530782,"name":"Yongheng Liang","orcid":"0000-0002-5430-2042","position":9,"is_corresponding":false},{"id":1320143,"name":"Zhiping Xie","orcid":"0009-0009-3218-1620","position":10,"is_corresponding":false},{"id":1543248,"name":"Zhong-Qiu Yu","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Dual roles of Atg8−PE deconjugation by Atg4 in autophagy","abstract":"Modification of target molecules by ubiquitin or ubiquitin-like (Ubl) proteins is generally reversible. Little is known, however, about the physiological function of the reverse reaction, deconjugation. Atg8 is a unique Ubl protein whose conjugation target is the lipid phosphatidylethanolamine (PE). Atg8 functions in the formation of double-membrane autophagosomes, a central step in the well-conserved intracellular degradation pathway of macroautophagy (hereafter autophagy). Here we show that the deconjugation of Atg8-PE by the cysteine protease Atg4 plays dual roles in the formation of autophagosomes. During the early stage of autophagosome formation, deconjugation releases Atg8 from non-autophagosomal membranes to maintain a proper supply of Atg8. At a later stage, the release of Atg8 from intermediate autophagosomal membranes facilitates the maturation of these structures into fusion-capable autophagosomes. These results provide new insights into the functions of Atg8-PE and its deconjugation.","is_dataset_classified":null,"base_score":5.484796933490655,"endowment":5.484796933490655,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"22652539","pmcid":"PMC3427254","openalex_id":"https://openalex.org/W2057813271","authors":[],"funders":[{"funder_name":"NIGMS NIH HHS","grant_id":"R01 GM053396","title":null},{"funder_name":"NIGMS NIH HHS","grant_id":"GM53396","title":null},{"funder_name":"National Institutes of Health","grant_id":"9R01GM053396-05","title":"ALTERNATIVE VACUOLAR TARGETING MECHANISMS IN YEAST"}],"total_grants":3,"fwci":7.9581,"citation_percentile":0.9814165,"influential_citations":0,"citation_trend":[{"year":2012,"count":1},{"year":2013,"count":12},{"year":2014,"count":15},{"year":2015,"count":13},{"year":2016,"count":13},{"year":2017,"count":28},{"year":2018,"count":16},{"year":2019,"count":19},{"year":2020,"count":31},{"year":2021,"count":28},{"year":2022,"count":16},{"year":2023,"count":13},{"year":2024,"count":15},{"year":2025,"count":17},{"year":2026,"count":3}],"oa_status":"hybrid","license":"cc-by-nc","oa_locations":[{"url":"https://www.tandfonline.com/doi/pdf/10.4161/auto.19652?needAccess=true","host_type":"journal"},{"url":"https://www.tandfonline.com/doi/pdf/10.4161/auto.19652?needAccess=true","host_type":"publisher"},{"url":"https://www.tandfonline.com/doi/pdf/10.4161/auto.19652","host_type":"publisher"},{"url":"https://doi.org/10.4161/auto.19652","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/22652539","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3427254","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC3427254","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC3427254?pdf=render","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/pmc3427254?pdf=render","host_type":""},{"url":"http://dx.doi.org/10.4161/auto.19652","host_type":""},{"url":"https://dx.doi.org/10.4161/auto.19652","host_type":""}],"fields_of_study":["Autophagy in Disease and Therapy","Endoplasmic Reticulum Stress and Disease","Toxoplasma gondii Research Studies","0301 basic medicine","0303 health sciences","03 medical and health sciences","Autophagy","Autophagy-Related Protein 8 Family","Autophagy-Related Proteins","Intracellular Membranes","Microtubule-Associated Proteins","Models, Biological","Phagosomes","Phosphatidylethanolamines","Saccharomyces cerevisiae","Saccharomyces cerevisiae Proteins","Vacuoles"],"mesh_terms":["Autophagy-Related Proteins","Autophagy-Related Protein 8 Family","Autophagy","Intracellular Membranes","Microtubule-Associated Proteins","Models, Biological","Phagosomes","Phosphatidylethanolamines","Saccharomyces cerevisiae","Vacuoles","Saccharomyces cerevisiae Proteins"],"keywords":["ATG8","Autophagy","Lipid-anchored protein","Cell biology","Autophagosome","Biology","Ubiquitin","Cysteine protease","Phosphatidylethanolamine","Membrane","Intracellular","Protease","Biochemistry","Enzyme","Phospholipid","Gene","Saccharomyces cerevisiae Proteins","Phosphatidylethanolamines","Basic Brief Report","Autophagy-Related Proteins","Autophagy-Related Protein 8 Family","Intracellular Membranes","Saccharomyces cerevisiae","Models, Biological","Phagosomes","Vacuoles","Microtubule-Associated Proteins"],"sdg_mappings":[{"sdg_number":3,"sdg_label":"3. 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