{"doi":"10.4155/bio-2023-0078","title":"An LC–MS/MS Method for Simultaneous Determination of LB-100 and Its Active Metabolite, Endothall, in Human Plasma","abstract":"We have developed and validated a novel LC–MS/MS method for the simultaneous quantification of LB-100 and its active metabolite, endothall, in human plasma following solid-phase extraction. LB-105 and endothall-D6 were used as internal standards. Chromatographic separation was achieved on a Hypercarb™ column using 5 mM (NH4)2CO3 and 30:70 (v/v) 100 mM (NH4)2CO3:acetonitrile as mobile phases. Detection was performed via positive electrospray ionization mode with multiple reaction monitoring. The assay exhibited linearity in the concentration range of 2.5–500 ng/ml for both analytes. Intra- and inter-assay precision and accuracy were within ±11%. LB-100 and endothall recoveries were 78.7 and 86.7%, respectively. The validated LC–MS/MS method enabled the accurate measurement of LB-100 and endothall in patient samples from an ongoing clinical trial (NCT04560972).","journal":"Bioanalysis","year":2023,"id":386593,"datarank":0.10397207708399181,"base_score":0.6931471805599453,"endowment":0.6931471805599453,"self_citation_contribution":0.10397207708399181,"citation_network_contribution":0.0,"self_endowment_contribution":0.10397207708399181,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9547,"is_data_producer":true,"deposit_databanks":{"ClinicalTrials.gov":["NCT04560972"]},"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":449643,"name":"Erminia Massarelli","orcid":"0000-0001-7798-7286","position":1,"is_corresponding":false},{"id":1155492,"name":"Eric Forman","orcid":null,"position":2,"is_corresponding":false},{"id":126954,"name":"John S. Kovach","orcid":null,"position":3,"is_corresponding":false},{"id":108575,"name":"Ravi Salgia","orcid":"0000-0001-9643-7626","position":4,"is_corresponding":false},{"id":392954,"name":"Timothy W. Synold","orcid":"0000-0002-4075-2544","position":5,"is_corresponding":false},{"id":805745,"name":"Ye Feng","orcid":"0000-0002-6551-1560","position":0,"is_corresponding":true}],"reference_count":19,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:18:00.717763Z","pmid":"37584370","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}