{"doi":"10.4155/bio-2020-0007","title":"A Robust LC–MS/MS Method for Amikacin: Application to Cellular Uptake and Pharmacokinetic Studies","abstract":"Background: Aminoglycosides are last-resort antibiotics for bacterial infections due to concerns of nephrotoxicity. A robust method is needed to correlate the magnitude of drug accumulation in the kidneys and the onset of nephrotoxicity. Materials & methods: A LC–MS/MS assay was developed, circumventing common limitations associated with conventional assays. To demonstrate its applicability, renal cellular uptake and rat pharmacokinetic studies were performed with amikacin. Results: To improve elution, the mobile phases were optimized with 60 mM ammonium hydroxide (pH = 11.2). An extended quantifiable range was achieved with different ionization modes. Kidney cells incubated with escalating amikacin concentrations showed increased uptake. Single-dose pharmacokinetics of amikacin were reasonably characterized. Conclusion: This assay will facilitate future studies on improving amikacin-associated nephrotoxicity.","journal":"Bioanalysis","year":2020,"id":86191,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":14,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9575,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":439779,"name":"Weiqun Wang","orcid":"0000-0002-1295-8246","position":1,"is_corresponding":false},{"id":440838,"name":"Kimberly R. Ledesma","orcid":null,"position":2,"is_corresponding":false},{"id":363040,"name":"Taijun Yin","orcid":null,"position":3,"is_corresponding":false},{"id":439780,"name":"Vincent H. Tam","orcid":"0000-0001-7071-5376","position":4,"is_corresponding":false},{"id":439778,"name":"Katrina Chan","orcid":"0000-0002-4589-2781","position":0,"is_corresponding":true}],"reference_count":15,"raw_metadata":null,"created_at":"2026-07-18T21:58:41.997408Z","pmid":"32343148","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}