{"doi":"10.4103/0972-9062.316273","title":"First molecular survey of Anaplasma phagocytophilum in hard ticks (Ixodidae) from Southwestern Iran","abstract":"<jats:sec>\n            <jats:title/>\n            <jats:p>\n                                 <jats:italic toggle=\"yes\">Anaplasma phagocytophilum</jats:italic> is a gram-negative obligate intracellular tick-borne rickettsia with veterinary and public health importance worldwide. This organism is an etiologic agent of tick-borne fever (TBF) in domesticated animals and human granulocytic anaplasmosis (HGA) as well. Hard ticks (Ixodida: Ixodidae) are incriminated as the main biologic vectors for <jats:italic toggle=\"yes\">Anaplasma</jats:italic> spp. Studies represent that <jats:italic toggle=\"yes\">Ixodes</jats:italic> spp. are the main vectors for <jats:italic toggle=\"yes\">A. phago-cytophilum</jats:italic> and few reports hinted that other tick species may play this role. So, the goal of the presented work was to investigate the <jats:italic toggle=\"yes\">A. phagocytophilum</jats:italic> in 2000 hard ticks in Khuzestan province of Iran by specific nested-PCR performing two consecutive amplifications of 16SrRNA gene fragment with highly variable nucleotide region. Each reaction included 10 salivary glands of distinct tick species. Specific nested-PCR on accumulated salivary glands detected specific bands in 15.5% of reactions (31 of 200) in electrophoresis only in <jats:italic toggle=\"yes\">Rhipicephalus sanguineous</jats:italic> and <jats:italic toggle=\"yes\">Hyalomma marginatum</jats:italic> ticks. We concluded that the broad distribution of <jats:italic toggle=\"yes\">A. phagocytophilum</jats:italic> infection is not only is due to the existence of <jats:italic toggle=\"yes\">Ixodes</jats:italic> spp. but other hard ticks may also play a role in this issue.</jats:p>\n          </jats:sec>","journal":"Journal of Vector Borne Diseases","year":2021,"id":664119,"datarank":0.19093899622777002,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.02614715292755356,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.02614715292755356,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":2,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1734050,"name":"Mohammad HosseinRazi Jallali","orcid":null,"position":1,"is_corresponding":false},{"id":1312058,"name":"Somayeh Bahrami","orcid":"0000-0002-2643-4494","position":2,"is_corresponding":false},{"id":348589,"name":"Mohammad Bagheri","orcid":"0000-0002-2764-7651","position":3,"is_corresponding":false},{"id":1734049,"name":"Hossein Hamidinejat","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"First molecular survey of Anaplasma phagocytophilum in hard ticks (Ixodidae) from Southwestern Iran","abstract":"<jats:sec>\n            <jats:title/>\n            <jats:p>\n                                 <jats:italic toggle=\"yes\">Anaplasma phagocytophilum</jats:italic> is a gram-negative obligate intracellular tick-borne rickettsia with veterinary and public health importance worldwide. This organism is an etiologic agent of tick-borne fever (TBF) in domesticated animals and human granulocytic anaplasmosis (HGA) as well. Hard ticks (Ixodida: Ixodidae) are incriminated as the main biologic vectors for <jats:italic toggle=\"yes\">Anaplasma</jats:italic> spp. Studies represent that <jats:italic toggle=\"yes\">Ixodes</jats:italic> spp. are the main vectors for <jats:italic toggle=\"yes\">A. phago-cytophilum</jats:italic> and few reports hinted that other tick species may play this role. So, the goal of the presented work was to investigate the <jats:italic toggle=\"yes\">A. phagocytophilum</jats:italic> in 2000 hard ticks in Khuzestan province of Iran by specific nested-PCR performing two consecutive amplifications of 16SrRNA gene fragment with highly variable nucleotide region. Each reaction included 10 salivary glands of distinct tick species. Specific nested-PCR on accumulated salivary glands detected specific bands in 15.5% of reactions (31 of 200) in electrophoresis only in <jats:italic toggle=\"yes\">Rhipicephalus sanguineous</jats:italic> and <jats:italic toggle=\"yes\">Hyalomma marginatum</jats:italic> ticks. We concluded that the broad distribution of <jats:italic toggle=\"yes\">A. phagocytophilum</jats:italic> infection is not only is due to the existence of <jats:italic toggle=\"yes\">Ixodes</jats:italic> spp. but other hard ticks may also play a role in this issue.</jats:p>\n          </jats:sec>","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"35074944","pmcid":null,"openalex_id":"https://openalex.org/W4210665161","authors":[],"funders":[],"total_grants":0,"fwci":0.1288,"citation_percentile":0.50870579,"influential_citations":0,"citation_trend":[{"year":2024,"count":2}],"oa_status":"gold","license":"cc-by-nc-sa","oa_locations":[{"url":"https://doi.org/10.4103/0972-9062.316273","host_type":"journal"},{"url":"https://doi.org/10.4103/0972-9062.316273","host_type":"publisher"},{"url":"https://journals.lww.com/10.4103/0972-9062.316273","host_type":"publisher"},{"url":"https://pubmed.ncbi.nlm.nih.gov/35074944","host_type":"repository"},{"url":"https://doaj.org/article/86444e92b76b4bf68af1cb454e3eba4b","host_type":"repository"}],"fields_of_study":["Viral Infections and Vectors","Vector-borne infectious diseases","Vector-Borne Animal Diseases","Anaplasma","Anaplasma phagocytophilum","Animals","Humans","Iran","Ixodidae","Tick-Borne Diseases"],"mesh_terms":["Anaplasma","Animals","Humans","Iran","Tick-Borne Diseases","Ixodidae","Anaplasma phagocytophilum"],"keywords":["Anaplasma phagocytophilum","Biology","Tick","Ixodidae","Ixodes scapularis","Ixodes","Obligate","Anaplasmosis","Anaplasma","Tick-borne disease","Dermacentor","Spotted fever","Rhipicephalus","Virology","Veterinary medicine","Zoology","Microbiology","Rickettsia","Ecology","Genetics","Borrelia burgdorferi","Medicine","Virus","Iran","Nested-PCR","Hard Ticks"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Peace, Justice and strong institutions"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-13T01:18:17.154485Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}