{"doi":"10.4049/jimmunol.90.2.185","title":"Neutralization of Visna Virus by Antisera from Sheep","abstract":"<jats:title>Summary</jats:title>\n               <jats:p>Neutralizing titers of visna antisera measured in tissue culture were found to increase with the time and the temperature at which serum-virus mixtures were incubated prior to inoculation into culture tubes. The omission of preincubation resulted in neutralizing titers about 50 to 100 times lower than those observed after preincubation at 37°C for 3 hr.</jats:p>\n               <jats:p>The rate of neutralization of visna virus by antiserum was found to depend on the temperature and on the antiserum concentration, the rate being greatly reduced at increased serum dilution.</jats:p>\n               <jats:p>An exponential linear relationship between the amount of virus neutralized and the antiserum titer was found for virus doses up to about 104.5. The neutralization line had a slope of about 4.0, a 10-fold increase in virus dose corresponding to a 1.8-fold decrease in serum titer.</jats:p>\n               <jats:p>A virus fraction of about 10-5 to 10-4 could not be neutralized either by raising the antiserum concentration or by increasing the time of incubation at 22°C. The nonneutralizable fraction was found to be present in undiluted serum-virus mixture and it was not significantly increased by dilution, indicating practically no reactivation of neutralized visna virus.</jats:p>","journal":"The Journal of Immunology","year":1963,"id":603014,"datarank":0.519860385419959,"base_score":3.4657359027997265,"endowment":3.4657359027997265,"self_citation_contribution":0.519860385419959,"citation_network_contribution":0.0,"self_endowment_contribution":0.519860385419959,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":31,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1546741,"name":"Halldór Thormar","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Neutralization of Visna Virus by Antisera from Sheep","abstract":"<jats:title>Summary</jats:title>\n               <jats:p>Neutralizing titers of visna antisera measured in tissue culture were found to increase with the time and the temperature at which serum-virus mixtures were incubated prior to inoculation into culture tubes. The omission of preincubation resulted in neutralizing titers about 50 to 100 times lower than those observed after preincubation at 37°C for 3 hr.</jats:p>\n               <jats:p>The rate of neutralization of visna virus by antiserum was found to depend on the temperature and on the antiserum concentration, the rate being greatly reduced at increased serum dilution.</jats:p>\n               <jats:p>An exponential linear relationship between the amount of virus neutralized and the antiserum titer was found for virus doses up to about 104.5. The neutralization line had a slope of about 4.0, a 10-fold increase in virus dose corresponding to a 1.8-fold decrease in serum titer.</jats:p>\n               <jats:p>A virus fraction of about 10-5 to 10-4 could not be neutralized either by raising the antiserum concentration or by increasing the time of incubation at 22°C. The nonneutralizable fraction was found to be present in undiluted serum-virus mixture and it was not significantly increased by dilution, indicating practically no reactivation of neutralized visna virus.</jats:p>","is_dataset_classified":null,"base_score":3.4657359027997265,"endowment":3.4657359027997265,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"13984956","pmcid":null,"openalex_id":"https://openalex.org/W1494372634","authors":[],"funders":[],"total_grants":0,"fwci":5.6491,"citation_percentile":0.95462185,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":"https://academic.oup.com/pages/standard-publication-reuse-rights","oa_locations":[{"url":"https://journals.aai.org/jimmunol/article-pdf/90/2/185/1478065/ji0900020185.pdf","host_type":"journal"},{"url":"https://journals.aai.org/jimmunol/article-pdf/90/2/185/1478065/ji0900020185.pdf","host_type":"publisher"},{"url":"https://academic.oup.com/jimmunol/article-pdf/90/2/185/62740538/ji0900020185.pdf","host_type":"publisher"},{"url":"https://doi.org/10.4049/jimmunol.90.2.185","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/13984956","host_type":"repository"}],"fields_of_study":["Virus-based gene therapy research","Viral gastroenteritis research and epidemiology","Viral Infectious Diseases and Gene Expression in Insects"],"mesh_terms":["Animals","Immune Sera","Sheep","Sheep Diseases","Virus Diseases","Visna-maedi virus","Sheep, Domestic"],"keywords":["Antiserum","Titer","Neutralization","Virus","Virology","Incubation","Dilution","Chemistry","Biology","Antibody","Immunology","Biochemistry","Virus diseases","Immune Serums","Sheep Diseases"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T20:53:32.889687Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}