{"doi":"10.4049/jimmunol.2300572","title":"CHTOP Promotes Microglia-Mediated Inflammation by Regulating Cell Metabolism and Inflammatory Gene Expression","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>During the initiation of the inflammatory response of microglia, the expression of many inflammation- and cell metabolism–related genes alters. However, how the transcription of inflammation- and metabolism-related genes are coordinately regulated during inflammation initiation is poorly understood. In this study, we found that LPS stimulation induced the expression of the chromatin target of PRMT1 (protein arginine methyltransferase 1) (CHTOP) in microglia. Knocking down CHTOP in microglia decreased proinflammatory cytokine expression. In addition, CHTOP knockdown altered cell metabolism, as both the upregulated genes were enriched in cell metabolism–related pathways and the metabolites profile was greatly altered based on untargeted metabolomics analysis. Mechanistically, CHTOP could directly bind the regulatory elements of inflammation and cell metabolism–related genes to regulate their transcription. In addition, knocking down CHTOP increased neuronal viability in vitro and alleviated microglia-mediated neuroinflammation in a systemic LPS treatment mouse model. Collectively, these data revealed CHTOP as a novel regulator to promote microglia-mediated neuroinflammation by coordinately regulating the transcription of inflammation and cell metabolism–related genes.</jats:p>","journal":"The Journal of Immunology","year":2024,"id":678569,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1772959,"name":"Mengfei Lv","orcid":null,"position":1,"is_corresponding":false},{"id":1772961,"name":"Zhongying Duan","orcid":"0009-0000-2809-4609","position":2,"is_corresponding":false},{"id":875026,"name":"Wenhao Liu","orcid":"0000-0002-2243-8391","position":3,"is_corresponding":false},{"id":514861,"name":"Feng Yan","orcid":"0000-0001-9926-7554","position":4,"is_corresponding":false},{"id":1772964,"name":"Jiake Liu","orcid":"0009-0002-7723-7789","position":5,"is_corresponding":false},{"id":1287581,"name":"Yu Cui","orcid":"0000-0002-1481-9389","position":6,"is_corresponding":false},{"id":280981,"name":"Xin Zhou","orcid":"0000-0003-4015-4787","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"CHTOP Promotes Microglia-Mediated Inflammation by Regulating Cell Metabolism and Inflammatory Gene Expression","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>During the initiation of the inflammatory response of microglia, the expression of many inflammation- and cell metabolism–related genes alters. However, how the transcription of inflammation- and metabolism-related genes are coordinately regulated during inflammation initiation is poorly understood. In this study, we found that LPS stimulation induced the expression of the chromatin target of PRMT1 (protein arginine methyltransferase 1) (CHTOP) in microglia. Knocking down CHTOP in microglia decreased proinflammatory cytokine expression. In addition, CHTOP knockdown altered cell metabolism, as both the upregulated genes were enriched in cell metabolism–related pathways and the metabolites profile was greatly altered based on untargeted metabolomics analysis. Mechanistically, CHTOP could directly bind the regulatory elements of inflammation and cell metabolism–related genes to regulate their transcription. In addition, knocking down CHTOP increased neuronal viability in vitro and alleviated microglia-mediated neuroinflammation in a systemic LPS treatment mouse model. Collectively, these data revealed CHTOP as a novel regulator to promote microglia-mediated neuroinflammation by coordinately regulating the transcription of inflammation and cell metabolism–related genes.</jats:p>","is_dataset_classified":null,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"38117276","pmcid":null,"openalex_id":"https://openalex.org/W4390016086","authors":[],"funders":[{"funder_name":"MOST | National Natural Science Foundation of China","grant_id":"31900634","title":null},{"funder_name":"山东省教育厅 | Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China","grant_id":"2022KJ146","title":null}],"total_grants":2,"fwci":0.6513,"citation_percentile":0.65810975,"influential_citations":0,"citation_trend":[{"year":2024,"count":1},{"year":2025,"count":5},{"year":2026,"count":1}],"oa_status":"closed","license":"https://academic.oup.com/pages/standard-publication-reuse-rights","oa_locations":[{"url":"https://academic.oup.com/jimmunol/article-pdf/212/4/677/61474989/ji2300572.pdf","host_type":"publisher"},{"url":"https://doi.org/10.4049/jimmunol.2300572","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/38117276","host_type":"repository"}],"fields_of_study":["Cancer-related gene regulation","Epigenetics and DNA Methylation","IL-33, ST2, and ILC Pathways"],"mesh_terms":["Neuroinflammatory Diseases","Animals","Inflammation","Lipopolysaccharides","Transcription Factors","Gene Expression","Microglia","Mice"],"keywords":["Microglia","Neuroinflammation","Inflammation","Cell biology","Proinflammatory cytokine","Biology","Gene knockdown","Transcription factor","Downregulation and upregulation","Gene expression","Immunology","Gene","Biochemistry"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-17T05:37:19.680189Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}