{"doi":"10.4049/jimmunol.2300166","title":"Single-Cell Analysis of CX3CR1+ Cells Reveals a Pathogenic Role for BIRC5+ Myeloid Proliferating Cells Driven by <i>Staphylococcus aureus</i> Leukotoxins","abstract":"Our previous studies identified a population of stem cell-like proliferating myeloid cells within inflamed tissues that could serve as a reservoir for tissue macrophages to adopt different activation states depending on the microenvironment. By lineage-tracing cells derived from CX3CR1+ precursors in mice during infection and profiling by single-cell RNA sequencing, in this study, we identify a cluster of BIRC5+ myeloid cells that expanded in the liver during chronic infection with either the parasite Schistosoma mansoni or the bacterial pathogen Staphylococcus aureus. In the absence of tissue-damaging toxins, S. aureus infection does not elicit these BIRC5+ cells. Moreover, deletion of BIRC5 from CX3CR1-expressing cells results in improved survival during S. aureus infection. Hence the combination of single-cell RNA sequencing and genetic fate-mapping CX3CR1+ cells revealed a toxin-dependent pathogenic role for BIRC5 in myeloid cells during S. aureus infection.","journal":"The Journal of Immunology","year":2023,"id":367180,"datarank":0.38773965876467653,"base_score":1.791759469228055,"endowment":1.791759469228055,"self_citation_contribution":0.26876392038420827,"citation_network_contribution":0.11897573838046828,"self_endowment_contribution":0.26876392038420827,"citer_contribution":0.11897573838046828,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":5,"citers_with_citation_signal":3,"citers_with_endowment":3,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9515,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":253551,"name":"Joseph C. Devlin","orcid":"0000-0002-0003-4812","position":1,"is_corresponding":false},{"id":478774,"name":"Keenan A. Lacey","orcid":"0000-0002-9242-0170","position":2,"is_corresponding":false},{"id":989502,"name":"Nina Howard","orcid":"0000-0002-1204-5885","position":3,"is_corresponding":false},{"id":671025,"name":"Ze Chen","orcid":"0000-0001-8236-5764","position":4,"is_corresponding":false},{"id":345071,"name":"Erin E. Zwack","orcid":"0000-0003-4729-8468","position":5,"is_corresponding":false},{"id":253549,"name":"Jian-Da Lin","orcid":"0000-0003-3697-4766","position":6,"is_corresponding":false},{"id":2201,"name":"Kelly V. Ruggles","orcid":"0000-0002-0152-0863","position":7,"is_corresponding":false},{"id":75650,"name":"Kamal M. Khanna","orcid":"0000-0002-9328-3817","position":8,"is_corresponding":false},{"id":308670,"name":"Victor J. Torres","orcid":"0000-0002-7126-0489","position":9,"is_corresponding":false},{"id":253555,"name":"P’ng Loke","orcid":"0000-0002-6211-3292","position":10,"is_corresponding":false},{"id":1124342,"name":"Denis G. Loredan","orcid":"0000-0001-7889-7776","position":0,"is_corresponding":true}],"reference_count":43,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T01:15:12.215513Z","pmid":"37466391","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}