{"doi":"10.4049/jimmunol.2200082","title":"Persistent Antigen Harbored by Alveolar Macrophages Enhances the Maintenance of Lung-Resident Memory CD8+ T Cells","abstract":"Abstract Lung tissue-resident memory T cells are crucial mediators of cellular immunity against respiratory viruses; however, their gradual decline hinders the development of T cell–based vaccines against respiratory pathogens. Recently, studies using adenovirus (Ad)-based vaccine vectors have shown that the number of protective lung-resident CD8+ TRMs can be maintained long term. In this article, we show that immunization of mice with a replication-deficient Ad serotype 5 expressing influenza (A/Puerto Rico/8/34) nucleoprotein (AdNP) generates a long-lived lung TRM pool that is transcriptionally indistinct from those generated during a primary influenza infection. In addition, we demonstrate that CD4+ T cells contribute to the long-term maintenance of AdNP-induced CD8+ TRMs. Using a lineage tracing approach, we identify alveolar macrophages as a cell source of persistent NP Ag after immunization with AdNP. Importantly, depletion of alveolar macrophages after AdNP immunization resulted in significantly reduced numbers of NP-specific CD8+ TRMs in the lungs and airways. Combined, our results provide further insight to the mechanisms governing the enhanced longevity of Ag-specific CD8+ lung TRMs observed after immunization with recombinant Ad.","journal":"The Journal of Immunology","year":2022,"id":256822,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":19,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9518,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":280877,"name":"Ida Uddbäck","orcid":"0000-0001-6007-5918","position":1,"is_corresponding":false},{"id":270265,"name":"Christopher D. Scharer","orcid":"0000-0001-7716-8504","position":2,"is_corresponding":false},{"id":302243,"name":"Mi Tian","orcid":"0000-0002-6446-1995","position":3,"is_corresponding":false},{"id":270264,"name":"Jeremy M. Boss","orcid":"0000-0002-2432-1840","position":4,"is_corresponding":false},{"id":280879,"name":"Allan Randrup Thomsen","orcid":"0000-0002-2392-9803","position":5,"is_corresponding":false},{"id":75428,"name":"Jan Pravsgaard Christensen","orcid":"0000-0002-4299-9479","position":6,"is_corresponding":false},{"id":232939,"name":"Jacob E. Kohlmeier","orcid":"0000-0002-7209-6641","position":7,"is_corresponding":false},{"id":232938,"name":"Jenna L Lobby","orcid":"0000-0002-1395-2583","position":0,"is_corresponding":true}],"reference_count":87,"raw_metadata":null,"created_at":"2026-07-19T00:25:25.728895Z","pmid":"36162870","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}