{"doi":"10.4049/jimmunol.2100884","title":"CD11bhigh B Cells Increase after Stroke and Regulate Microglia","abstract":"Abstract Recent studies have highlighted the deleterious contributions of B cells to post-stroke recovery and cognitive decline. Different B cell subsets have been proposed on the basis of expression levels of transcription factors (e.g., T-bet) as well as specific surface proteins. CD11b (α-chain of integrin) is expressed by several immune cell types and is involved in regulation of cell motility, phagocytosis, and other essential functions of host immunity. Although B cells express CD11b, the CD11bhigh subset of B cells has not been well characterized, especially in immune dysregulation seen with aging and after stroke. Here, we investigate the role of CD11bhigh B cells in immune responses after stroke in young and aged mice. We evaluated the ability of CD11bhigh B cells to influence pro- and anti-inflammatory phenotypes of young and aged microglia (MG). We hypothesized that CD11bhigh B cells accumulate in the brain and contribute to neuroinflammation in aging and after stroke. We found that CD11bhigh B cells are a heterogeneous subpopulation of B cells predominantly present in naive aged mice. Their frequency increases in the brain after stroke in young and aged mice. Importantly, CD11bhigh B cells regulate MG phenotype and increase MG phagocytosis in both ex vivo and in vivo settings, likely by production of regulatory cytokines (e.g., TNF-α). As both APCs and adaptive immune cells with long-term memory function, B cells are uniquely positioned to regulate acute and chronic phases of the post-stroke immune response, and their influence is subset specific.","journal":"The Journal of Immunology","year":2022,"id":246131,"datarank":0.5676284450877392,"base_score":3.784189633918261,"endowment":3.784189633918261,"self_citation_contribution":0.5676284450877392,"citation_network_contribution":0.0,"self_endowment_contribution":0.5676284450877392,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":43,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.946,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":229318,"name":"Pedram Honarpisheh","orcid":null,"position":1,"is_corresponding":false},{"id":881897,"name":"Eric C. Mohan","orcid":"0000-0003-4950-9716","position":2,"is_corresponding":false},{"id":272494,"name":"Anik Banerjee","orcid":"0000-0003-4369-2628","position":3,"is_corresponding":false},{"id":272495,"name":"Maria P. Blasco-Conesa","orcid":"0000-0002-0700-4907","position":4,"is_corresponding":false},{"id":272496,"name":"Parisa Honarpisheh","orcid":"0000-0002-8902-7976","position":5,"is_corresponding":false},{"id":881898,"name":"Gary Guzman","orcid":"0000-0001-8002-9994","position":6,"is_corresponding":false},{"id":881899,"name":"Romeesa Khan","orcid":"0000-0002-4129-1612","position":7,"is_corresponding":false},{"id":225944,"name":"Bhanu Priya Ganesh","orcid":"0000-0003-1575-7914","position":8,"is_corresponding":false},{"id":410765,"name":"Amy L. Hazen","orcid":null,"position":9,"is_corresponding":false},{"id":90492,"name":"Juneyoung Lee","orcid":"0000-0002-9805-7835","position":10,"is_corresponding":false},{"id":460685,"name":"Aditya Kumar","orcid":"0000-0003-1096-712X","position":11,"is_corresponding":false},{"id":104957,"name":"Louise D. McCullough","orcid":"0000-0002-8050-1686","position":12,"is_corresponding":false},{"id":272498,"name":"Anjali Chauhan","orcid":"0000-0002-9825-8100","position":13,"is_corresponding":false},{"id":881896,"name":"Janelle Korf","orcid":"0000-0003-0246-6894","position":0,"is_corresponding":true}],"reference_count":87,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T00:23:43.438539Z","pmid":"35732342","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}