{"doi":"10.4049/jimmunol.210.supp.60.04","title":"Single cell transcriptomics identify AHR and CD9 as markers of CD14+ atypical B cells","abstract":"Abstract B lymphocytes are adaptive immune cells responsible for the production of antigen specific antibodies and the release of pro- and anti-inflammatory cytokines. Pro-inflammatory B cells, often termed atypical B cells (at B cell) are a heterogenous population of B lymphocytes associated with aging, infection, and autoimmunity. Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor important for xenobiotic metabolism. However, AHR is heavily implicated in immune cell differentiation as a regulator of cell fate decisions. at B cells are believed to represent an alternative B cell lineage but the role of AHR in at B cells is not known. We have reported that human, primary CD5+ innate-like B cells (ILB) are preferentially sensitive to modulation by AHR-activation compared to CD5− B cells. CD5+ ILB are a heterogenous population of B cells enriched in IgM memory B cells, B1 B cells, marginal zone B cells, regulatory B cells, and at B cells. We used Single cell transcriptomics to elucidate AHR expression in human naïve, circulating CD19+ B cells isolated directly from PBMC and enriched for CD5 protein. We found that AHR expression correlated with expression of CD14 and were enriched in a myeloid gene signature. These results were validated in isolated CD9+ B cells and PBMC. Further, CD14 expressing B cells were enriched in CD9 isolated cells and expressed higher CD11c and Tbet and lower IgD, markers of atypical B cells, compared to CD9− B cells. These data suggest that CD9 and AHR are putative markers of CD14+ at B cells. (Supported by NIH grant P42ES004911) Supported by NIH grant P42ES004911","journal":"The Journal of Immunology","year":2023,"id":410209,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.956,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1190316,"name":"Isha O Khan","orcid":null,"position":1,"is_corresponding":false},{"id":666082,"name":"Robert B. Crawford","orcid":"0000-0002-8507-1021","position":2,"is_corresponding":false},{"id":666083,"name":"Norbert E. Kaminski","orcid":"0000-0002-2144-428X","position":3,"is_corresponding":false},{"id":593507,"name":"Lance K. Blevins","orcid":"0000-0002-8911-8843","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T01:21:31.144858Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}