{"doi":"10.4049/jimmunol.208.supp.53.02","title":"Secreted IgM modulates the pool of IL-10 producing B Cells","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Regulatory B cells (Bregs) are critical for maintaining immune tolerance, and alterations in Breg numbers has detrimental effects in inflammatory diseases, infection, and cancer. Bregs are found among most B cell subsets and act primarily by producing IL-10. Despite their importance, the signals that control Breg differentiation and maintenance are not well defined. Here, we demonstrate that mice incapable of secreting IgM (sIgM−/−) have drastically increased IL-10+ Bregs in lymphoid organs compared to wildtype (WT) mice. These IL-10+ Bregs are polyclonal and increased among all major B cell subsets. While newborn WT mice (&amp;lt;24 hr of age) harbor detectable serum IgM, IL-10+ B cell expansion occurs early after birth in both WT and sIgM−/− mice. However, IL-10+ Bregs contract only in WT but not in sIgM−/− mice, which is in parallel to rising levels of serum IgM in WT mice. SIgM−/− B cells that develop in the presence of circulating IgM express IL-10 similar to that of WT B cells within the same mouse indicating that the expansions in IL-10+ B cell populations are not due to B cell-intrinsic effects based on the inability to secrete IgM, but rather the presence of sIgM. Mice that lack B cell-expressed high-affinity IgM receptor (FcμR) mirror the IL-10 phenotype in their B-1 and marginal zone B cells, but not follicular B cells, suggesting that secreted IgM can act as a negative regulator of IL-10 expression in B cell subsets via binding to surface-expressed FcμR, and potentially other IgM binding receptors. Our data indicate that sIgM controls IL-10 competence in B cells via IgM binding receptors thereby revealing potential novel avenues for altering IL-10+ Breg induction in disease settings. Funding: T32AI134646, R01AR067751, R01AI127389, R01AI148652.</jats:p>\n               <jats:p>Supported by grants from NIH (T32AI134646, R01AR067751, R01AI127389, R01AI148652)</jats:p>","journal":"The Journal of Immunology","year":2022,"id":45883,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":213610,"name":"Lazaro E Aira","orcid":null,"position":1,"is_corresponding":false},{"id":213611,"name":"Gaurav Kumar","orcid":null,"position":2,"is_corresponding":false},{"id":213612,"name":"Nicole Baumgarth","orcid":null,"position":3,"is_corresponding":false},{"id":213613,"name":"Gudrun F Debes","orcid":null,"position":4,"is_corresponding":false},{"id":213609,"name":"Shannon E McGettigan","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"21071399","pmcid":null,"openalex_id":"https://openalex.org/W4313404621","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.19039046,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"https://academic.oup.com/pages/standard-publication-reuse-rights","oa_locations":[{"url":"https://academic.oup.com/jimmunol/article/208/Supplement_1/53.02/7940578","host_type":"publisher"},{"url":"https://doi.org/10.4049/jimmunol.208.supp.53.02","host_type":"journal"}],"fields_of_study":["T-cell and B-cell Immunology","Immune Cell Function and Interaction","Monoclonal and Polyclonal Antibodies Research","Biology","Medicine"],"mesh_terms":[],"keywords":["Regulatory B cells","B cell","Biology","Immunology","Interleukin 10","Marginal zone","Naive B cell","Secretion","Immune system","Immunoglobulin M","Antibody","Molecular biology","T cell","Immunoglobulin G","Endocrinology","Antigen-presenting cell"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-04T18:58:42.489360Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}