{"doi":"10.4049/jimmunol.2000784","title":"Non–Cell-Autonomous Activity of the Hemidesmosomal Protein BP180/Collagen XVII in Granulopoiesis in Humanized NC16A Mice","abstract":"Abstract BP180 (also termed type XVII collagen) is a hemidesmosomal protein and plays a critical role in cell–cell matrix adhesion in the skin; however, its other biological functions are largely unclear. In this study, we generated a BP180 functional–deficient mouse strain by deleting its extracellular domain of humanized NC16A (termed ΔNC16A mice). We found that BP180 is expressed by bone marrow mesenchymal stem cells (BM-MSC), and its functional deficiency leads to myeloid hyperplasia. Altered granulopoiesis in ΔNC16A mice is through bone marrow stromal cells evidenced by bone marrow transplantation. Furthermore, the level of G-CSF in bone marrow and circulation were significantly increased in ΔNC16A mice as compared with wild-type mice. The increased G-CSF was accompanied by an increased activation of the NF-κB signaling pathway in bone marrow and BM-MSC of ΔNC16A mice. Blockade of G-CSF restored normal granulopoiesis in ΔNC16A mice. Inhibition of NF-κB signaling pathway significantly reduces the release of G-CSF from ΔNC16A BM-MSC in vitro and the level of serum G-CSF in ΔNC16A mice. To our knowledge, these findings provide the first direct evidence that BP180 plays an important role in granulopoiesis through regulating NF-κB signaling pathway in BM-MSC.","journal":"The Journal of Immunology","year":2020,"id":111938,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9513,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":530458,"name":"Bin-Jin Hwang","orcid":"0009-0001-7623-9240","position":1,"is_corresponding":false},{"id":528941,"name":"Ning Li","orcid":"0000-0003-0185-983X","position":2,"is_corresponding":false},{"id":530459,"name":"Paul B. Googe","orcid":"0000-0002-9185-3548","position":3,"is_corresponding":false},{"id":419926,"name":"Luis A. Diaz","orcid":"0000-0002-7308-292X","position":4,"is_corresponding":false},{"id":293211,"name":"Edward A. Miao","orcid":"0000-0001-7295-3490","position":5,"is_corresponding":false},{"id":275084,"name":"Barbara J. Vilen","orcid":"0000-0003-0014-0022","position":6,"is_corresponding":false},{"id":388155,"name":"Nancy E. Thomas","orcid":"0000-0002-2230-1328","position":7,"is_corresponding":false},{"id":299129,"name":"Jenny P.‐Y. Ting","orcid":"0000-0002-7846-0395","position":8,"is_corresponding":false},{"id":530460,"name":"Zhi Liu","orcid":"0000-0001-9785-9150","position":9,"is_corresponding":false},{"id":530457,"name":"Lin Lin","orcid":"0009-0008-8181-8758","position":0,"is_corresponding":true}],"reference_count":73,"raw_metadata":null,"created_at":"2026-07-18T23:13:09.353152Z","pmid":"32998984","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}