{"doi":"10.4049/jimmunol.190.supp.110.3","title":"Immunodominant and subdominant WNV epitopes are differentially presented (P5005)","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>There are many factors that are thought to contribute to T-cell immunodominance hierarchies including timing and levels of antigen presentation to T-cells. One of the most potent tools for studying antigen presentation for a specific determinant are monoclonal antibodies that are specific for not only MHC but a specific ligand, similar to a T cell receptor. In this study we utilize these antibodies to study levels and timing of antigen presentation of immunodominant (SVG9), sub-dominant (SLF9), and super sub-dominant (YTM9) T-cell epitopes during West Nile virus (WNV) infection. In this study, we demonstrate that these ligands are differentially expressed on the surface of a cell infected with WNV. Specifically, the super sub-dominant ligand (YTM9) was presented earlier and at 20 times the levels of the immunodominant (SVG9) and subdominant (SLF9) ligands. Transfection of ICP47 in the infected cells show that all three epitopes are TAP dependent. Using these highly specific reagents we show that there is differential ligand presentation within the restrictive allele. High levels of peptide HLA complexes on the surface of an infected do not correspond with immunodominance hierarchies as the super subdominant epitope YTM9 was presented at higher levels than the most dominant epitope SVG9. This differential in presentation was not due the TAP dependence of the ligands.</jats:p>","journal":"The Journal of Immunology","year":2013,"id":646182,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":457462,"name":"Sojung Kim","orcid":"0000-0002-8244-1613","position":1,"is_corresponding":false},{"id":605197,"name":"Wilfried Bardet","orcid":null,"position":2,"is_corresponding":false},{"id":605196,"name":"Fredda Schafer","orcid":null,"position":3,"is_corresponding":false},{"id":992490,"name":"Michael Diamond","orcid":"0000-0003-2147-5921","position":4,"is_corresponding":false},{"id":1682928,"name":"Jon Weidanz","orcid":null,"position":5,"is_corresponding":false},{"id":1535202,"name":"Ted Hansen","orcid":null,"position":6,"is_corresponding":false},{"id":1535205,"name":"William Hildebrand","orcid":null,"position":7,"is_corresponding":false},{"id":881592,"name":"Curtis McMurtrey","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Immunodominant and subdominant WNV epitopes are differentially presented (P5005)","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>There are many factors that are thought to contribute to T-cell immunodominance hierarchies including timing and levels of antigen presentation to T-cells. One of the most potent tools for studying antigen presentation for a specific determinant are monoclonal antibodies that are specific for not only MHC but a specific ligand, similar to a T cell receptor. In this study we utilize these antibodies to study levels and timing of antigen presentation of immunodominant (SVG9), sub-dominant (SLF9), and super sub-dominant (YTM9) T-cell epitopes during West Nile virus (WNV) infection. In this study, we demonstrate that these ligands are differentially expressed on the surface of a cell infected with WNV. Specifically, the super sub-dominant ligand (YTM9) was presented earlier and at 20 times the levels of the immunodominant (SVG9) and subdominant (SLF9) ligands. Transfection of ICP47 in the infected cells show that all three epitopes are TAP dependent. Using these highly specific reagents we show that there is differential ligand presentation within the restrictive allele. High levels of peptide HLA complexes on the surface of an infected do not correspond with immunodominance hierarchies as the super subdominant epitope YTM9 was presented at higher levels than the most dominant epitope SVG9. This differential in presentation was not due the TAP dependence of the ligands.</jats:p>","is_dataset_classified":null,"base_score":0.0,"endowment":0.0,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":null,"pmcid":null,"openalex_id":null,"authors":[],"funders":[],"total_grants":0,"fwci":null,"citation_percentile":null,"influential_citations":0,"citation_trend":[],"oa_status":null,"license":"https://academic.oup.com/pages/standard-publication-reuse-rights","oa_locations":[{"url":"https://academic.oup.com/jimmunol/article/190/Supplement_1/110.3/7999282","host_type":"publisher"}],"fields_of_study":[],"mesh_terms":[],"keywords":[],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-09T12:02:33.848940Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}