{"doi":"10.4049/jimmunol.1801085","title":"Human Anti–HIV-1 gp120 Monoclonal Antibodies with Neutralizing Activity Cloned from Humanized Mice Infected with HIV-1","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Broadly neutralizing, anti–HIV-1 gp120 mAbs have been isolated from infected individuals, and there is considerable interest in developing these reagents for Ab-based immunoprophylaxis and treatment. As a means to identify potentially new anti-HIV Abs, we exploited humanized NOD-scid IL2rγnull mice systemically infected with HIV-1 to generate a wide variety of Ag-specific human mAbs. The Abs were encoded by a diverse range of variable gene families and Ig classes, including IgA, and several showed significant levels of somatic mutation. Moreover, the isolated Abs not only bound target Ags with similar affinity as broadly neutralizing Abs, they also demonstrated neutralizing ability against multiple HIV-1 clades. The use of humanized mice will allow us to use our knowledge of HIV-1 gp120 structure and function, and the immune response targeting this protein, to generate native human prophylactic Abs to reduce the infection and spread of HIV-1.</jats:p>","journal":"The Journal of Immunology","year":2019,"id":687246,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1516121,"name":"Mark Duval","orcid":null,"position":1,"is_corresponding":false},{"id":551368,"name":"Claudia Carbone","orcid":"0000-0002-2420-8629","position":2,"is_corresponding":false},{"id":286938,"name":"Smita Jaiswal","orcid":null,"position":3,"is_corresponding":false},{"id":249885,"name":"Aaron Wallace","orcid":"0000-0003-0876-661X","position":4,"is_corresponding":false},{"id":1795416,"name":"Joseph C Martin","orcid":null,"position":5,"is_corresponding":false},{"id":550566,"name":"Ann Dauphin","orcid":"0000-0002-3570-8610","position":6,"is_corresponding":false},{"id":263551,"name":"Michael A. Brehm","orcid":"0000-0001-6813-3262","position":7,"is_corresponding":false},{"id":1795417,"name":"Dale L Greiner","orcid":null,"position":8,"is_corresponding":false},{"id":1795419,"name":"Leonard D Shultz","orcid":null,"position":9,"is_corresponding":false},{"id":284590,"name":"Jeremy Luban","orcid":"0000-0001-5650-4054","position":10,"is_corresponding":false},{"id":249894,"name":"Lisa A. Cavacini","orcid":"0000-0003-1417-8339","position":11,"is_corresponding":false},{"id":1795413,"name":"Melissa A Gawron","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Human Anti–HIV-1 gp120 Monoclonal Antibodies with Neutralizing Activity Cloned from Humanized Mice Infected with HIV-1","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Broadly neutralizing, anti–HIV-1 gp120 mAbs have been isolated from infected individuals, and there is considerable interest in developing these reagents for Ab-based immunoprophylaxis and treatment. As a means to identify potentially new anti-HIV Abs, we exploited humanized NOD-scid IL2rγnull mice systemically infected with HIV-1 to generate a wide variety of Ag-specific human mAbs. The Abs were encoded by a diverse range of variable gene families and Ig classes, including IgA, and several showed significant levels of somatic mutation. Moreover, the isolated Abs not only bound target Ags with similar affinity as broadly neutralizing Abs, they also demonstrated neutralizing ability against multiple HIV-1 clades. The use of humanized mice will allow us to use our knowledge of HIV-1 gp120 structure and function, and the immune response targeting this protein, to generate native human prophylactic Abs to reduce the infection and spread of HIV-1.</jats:p>","is_dataset_classified":null,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"30593536","pmcid":"PMC6344273","openalex_id":"https://openalex.org/W2906864007","authors":[],"funders":[{"funder_name":"NIDA NIH HHS","grant_id":"DP1 DA034990","title":null},{"funder_name":"NIDDK NIH HHS","grant_id":"UC4 DK104218","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI111809","title":null},{"funder_name":"NIH HHS","grant_id":"R24 OD018259","title":null},{"funder_name":"NCI NIH HHS","grant_id":"P30 CA034196","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI106478","title":null},{"funder_name":"NIAID NIH HHS","grant_id":"R01 AI132963","title":null},{"funder_name":"NIH HHS","grant_id":"R24 OD026440","title":null}],"total_grants":8,"fwci":0.3482,"citation_percentile":0.53734147,"influential_citations":0,"citation_trend":[{"year":2019,"count":1},{"year":2020,"count":2},{"year":2023,"count":2},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"green","license":"https://academic.oup.com/pages/standard-publication-reuse-rights","oa_locations":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6344273","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/6344273","host_type":"repository"},{"url":"https://academic.oup.com/jimmunol/article-pdf/202/3/799/61871636/ji1801085.pdf","host_type":"publisher"},{"url":"https://doi.org/10.4049/jimmunol.1801085","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/30593536","host_type":"repository"},{"url":"https://escholarship.umassmed.edu/pmm_pp/108","host_type":""},{"url":"https://mouseion.jax.org/stfb2019/18","host_type":"repository"}],"fields_of_study":["HIV Research and Treatment","Immune Cell Function and Interaction","T-cell and B-cell Immunology","Animals","Animals, Genetically Modified","Antibodies, Monoclonal, Humanized","Antibodies, Neutralizing","HIV Antibodies","HIV Envelope Protein gp120","HIV Infections","HIV-1","Humans","Mice","Mice, Inbred NOD","Mice, SCID","Neutralization Tests"],"mesh_terms":["Animals","Humans","Neutralization Tests","HIV Antibodies","HIV-1","HIV Infections","HIV Envelope Protein gp120","Mice, SCID","Mice, Inbred NOD","Animals, Genetically Modified","Mice","Antibodies, Neutralizing","Antibodies, Monoclonal, Humanized"],"keywords":["Humanized mouse","Virology","Monoclonal antibody","Humanized antibody","Neutralizing antibody","Antibody","Biology","Human immunodeficiency virus (HIV)","Immune system","Immunology","Virus"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-18T21:37:08.643224Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}