{"doi":"10.4049/jimmunol.173.3.1658","title":"Differential Roles for Wiskott-Aldrich Syndrome Protein in Immune Synapse Formation and IL-2 Production","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Wiskott-Aldrich syndrome protein (WASP)-deficient T cells exhibit defects in IL-2 production that are widely believed to stem from primary defects in actin remodeling and immune synapse formation. Surprisingly, however, we find that WASP-deficient T cells responding to Ag-specific APCs polymerize actin and organize talin and PKCθ normally, forming an immune synapse that is stable for at least 3 h. At low doses of peptide, WASP-deficient T cells show less efficient talin and PKCθ polarization. Thus, although WASP may facilitate immune synapse formation at low peptide concentrations, WASP is not required for this process. Defects in IL-2 production are observed even under conditions in which immune synapse formation proceeds normally, suggesting that the role of WASP in regulating IL-2 production is independent of its role in immune synapse formation.</jats:p>","journal":"The Journal of Immunology","year":2004,"id":596593,"datarank":0.7181237614173069,"base_score":4.787491742782046,"endowment":4.787491742782046,"self_citation_contribution":0.7181237614173069,"citation_network_contribution":0.0,"self_endowment_contribution":0.7181237614173069,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":119,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1527986,"name":"Janis K Burkhardt","orcid":null,"position":1,"is_corresponding":false},{"id":1527985,"name":"Judy L Cannon","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Differential Roles for Wiskott-Aldrich Syndrome Protein in Immune Synapse Formation and IL-2 Production","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Wiskott-Aldrich syndrome protein (WASP)-deficient T cells exhibit defects in IL-2 production that are widely believed to stem from primary defects in actin remodeling and immune synapse formation. Surprisingly, however, we find that WASP-deficient T cells responding to Ag-specific APCs polymerize actin and organize talin and PKCθ normally, forming an immune synapse that is stable for at least 3 h. At low doses of peptide, WASP-deficient T cells show less efficient talin and PKCθ polarization. Thus, although WASP may facilitate immune synapse formation at low peptide concentrations, WASP is not required for this process. Defects in IL-2 production are observed even under conditions in which immune synapse formation proceeds normally, suggesting that the role of WASP in regulating IL-2 production is independent of its role in immune synapse formation.</jats:p>","is_dataset_classified":null,"base_score":4.787491742782046,"endowment":4.787491742782046,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"15265894","pmcid":null,"openalex_id":"https://openalex.org/W1900620157","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"R01-AI44835","title":null}],"total_grants":1,"fwci":3.4291,"citation_percentile":0.92246405,"influential_citations":0,"citation_trend":[{"year":2012,"count":7},{"year":2013,"count":9},{"year":2014,"count":5},{"year":2015,"count":7},{"year":2016,"count":5},{"year":2018,"count":3},{"year":2019,"count":5},{"year":2020,"count":3},{"year":2021,"count":3},{"year":2022,"count":3},{"year":2023,"count":5},{"year":2024,"count":1},{"year":2025,"count":2}],"oa_status":"bronze","license":"https://academic.oup.com/pages/standard-publication-reuse-rights","oa_locations":[{"url":"https://journals.aai.org/jimmunol/article-pdf/173/3/1658/1186897/1658.pdf","host_type":"journal"},{"url":"https://journals.aai.org/jimmunol/article-pdf/173/3/1658/1186897/1658.pdf","host_type":"publisher"},{"url":"https://academic.oup.com/jimmunol/article-pdf/173/3/1658/62610306/1658.pdf","host_type":"publisher"},{"url":"https://doi.org/10.4049/jimmunol.173.3.1658","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/15265894","host_type":"repository"}],"fields_of_study":["Cellular Mechanics and Interactions","Cell Adhesion Molecules Research","T-cell and B-cell Immunology","Actins","Animals","Antigen Presentation","Cell Communication","Cells, Cultured","Cytochromes c","Cytotoxicity, Immunologic","DNA-Binding Proteins","Histocompatibility Antigens Class II","Intercellular Junctions","Interleukin-2","Isoenzymes","Mice","Mice, Inbred C3H","Mice, Knockout","Mice, Transgenic","Moths","NFATC Transcription Factors","Nuclear Proteins","Peptide Fragments","Protein Kinase C","Protein Kinase C-theta","Proteins","Receptors, Antigen, T-Cell","T-Lymphocyte Subsets","Talin","Transcription Factors","Wiskott-Aldrich Syndrome Protein"],"mesh_terms":["Protein Kinase C-theta","Actins","Animals","Histocompatibility Antigens Class II","Cell Communication","Cells, Cultured","Cytotoxicity, Immunologic","DNA-Binding Proteins","Intercellular Junctions","Interleukin-2","Isoenzymes","Mice, Inbred C3H","Mice, Transgenic","Moths","Nuclear Proteins","Peptide Fragments","Protein Kinase C","Proteins","Receptors, Antigen, T-Cell","Transcription Factors","T-Lymphocyte Subsets","Talin","Antigen Presentation","Mice, Knockout","Cytochromes c","NFATC Transcription Factors","Wiskott-Aldrich Syndrome Protein","Mice"],"keywords":["Immunological synapse","Wiskott–Aldrich syndrome protein","Immune system","Synapse","Cell biology","Actin","Biology","Wiskott–Aldrich syndrome","T cell","Immunology","Cytoskeleton","Actin cytoskeleton","T-cell receptor","Neuroscience","Cell","Genetics","Gene"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-28T10:44:22.855674Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}