{"doi":"10.4049/jimmunol.164.5.2281","title":"Cutting Edge: Complement-Activating Complex of Ficolin and Mannose-Binding Lectin-Associated Serine Protease","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>Both ficolins and mannose-binding lectin (MBL) are lectins characterized by the presence of collagen-like and carbohydrate-binding domains in a subunit, although their carbohydrate-binding moieties are quite different. A fibrinogen-like domain is in ficolins, and a carbohydrate recognition domain is in MBL. On binding to pathogens, human MBL activates the complement system via the lectin pathway in association with two types of MBL-associated serine proteases (MASP), MASP-1 and MASP-2 and its truncated form, small MBL-associated protein (sMAP, also called MAp19). We report here that ficolin/P35, a human serum ficolin, was found to copurify with MASPs and sMAP. MASPs that were complexed with ficolin/P35 exhibited proteolytic activities against complement components C4, C2, and C3. The ficolin/P35-MASPs-sMAP complex that was bound to Salmonella typhimurium activated complement. These findings indicate that ficolin/P35 is a second collagenous lectin capable of activating the lectin pathway and thus plays a role in innate immunity.</jats:p>","journal":"The Journal of Immunology","year":2000,"id":16953,"datarank":13.535799743864928,"base_score":5.793013608384144,"endowment":5.793013608384144,"self_citation_contribution":0.8689520412576217,"citation_network_contribution":12.666847702607306,"self_endowment_contribution":0.8689520412576217,"citer_contribution":12.666847702607306,"corpus_percentile":null,"corpus_rank":null,"citation_count":327,"citer_count":200,"citers_with_citation_signal":200,"citers_with_endowment":200,"datacite_reuse_total":25,"is_dataset":false,"is_dataset_confidence":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":123106,"name":"Yuichi Endo","orcid":null,"position":1,"is_corresponding":false},{"id":122928,"name":"Teizo Fujita","orcid":null,"position":2,"is_corresponding":false},{"id":122927,"name":"Misao Matsushita","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":5.793013608384144,"endowment":5.793013608384144,"datacite_reuse_total":25,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"10679061","pmcid":null,"openalex_id":"https://openalex.org/W1660694569","authors":[],"funders":[],"total_grants":0,"fwci":5.9084,"citation_percentile":0.97085251,"influential_citations":8,"citation_trend":[{"year":2012,"count":19},{"year":2013,"count":18},{"year":2014,"count":12},{"year":2015,"count":15},{"year":2016,"count":8},{"year":2017,"count":8},{"year":2018,"count":10},{"year":2019,"count":8},{"year":2020,"count":8},{"year":2021,"count":8},{"year":2022,"count":9},{"year":2023,"count":7},{"year":2024,"count":6},{"year":2025,"count":4},{"year":2026,"count":1}],"oa_status":"bronze","license":"https://academic.oup.com/pages/standard-publication-reuse-rights","oa_locations":[{"url":"https://journals.aai.org/jimmunol/article-pdf/164/5/2281/1315172/2281.pdf","host_type":"journal"},{"url":"https://journals.aai.org/jimmunol/article-pdf/164/5/2281/1315172/2281.pdf","host_type":"BRONZE"},{"url":"https://journals.aai.org/jimmunol/article-pdf/164/5/2281/1315172/2281.pdf","host_type":"publisher"},{"url":"https://academic.oup.com/jimmunol/article-pdf/164/5/2281/62140004/2281.pdf","host_type":"publisher"},{"url":"https://doi.org/10.4049/jimmunol.164.5.2281","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/10679061","host_type":"repository"}],"fields_of_study":["Invertebrate Immune Response Mechanisms","Complement system in diseases","Aquaculture disease management and microbiota","Chemistry","Medicine","Biology","Biopolymers","Carrier Proteins","Collectins","Complement Activation","Complement C1q","Complement C3","Enzyme Activation","Humans","Lectins","Mannose-Binding Protein-Associated Serine Proteases","Precipitin Tests","Serine Endopeptidases","Ficolins"],"mesh_terms":["Ficolins","Biopolymers","Carrier Proteins","Complement Activation","Complement C3","Enzyme Activation","Humans","Precipitin Tests","Serine Endopeptidases","Complement C1q","Lectins","Collectins","Mannose-Binding Protein-Associated Serine Proteases"],"keywords":["Mannan-binding lectin","Ficolin","MASP1","Lectin pathway","Lectin","Serine protease","Complement system","Serine","Complement (music)","Chemistry","Mannose","Biochemistry","Biology","Protease","Immunology","Classical complement pathway","Antibody","Gene","Enzyme"],"sdg_mappings":[],"linked_datasets":[{"doi":"10.6084/m9.figshare.13647076.v1","title":"Additional file 1 of The clinical value of ficolin-3 gene polymorphism in rheumatic heart disease. 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