{"doi":"10.4049/jimmunol.160.6.2655","title":"A Role for Perforin in Activation-Induced Cell Death","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>The granule exocytosis pathway of T cell cytotoxicity is absent in mice whose perforin gene has been ablated by targeted mutagenesis. The ability of activated naive T cells to undergo apoptosis in vitro following reaggregation of the TCR complex with anti-TCR mAbs via a Fas-independent pathway was found to be defective in the absence of perforin. Protection from death was most marked in CD8+ T cells. In wild-type cells, perforin was expressed at the same time that apoptosis occurred, and blockade of perforin expression by either incubation with perforin antisense oligonucleotides or with anti-IL-2 Abs resulted in increased viability of activated T cells. The role of perforin was not via perforin-dependent fratricidal killing. The results suggest a model in which perforin acts internally to cause a form of activation-induced T cell death distinct from that caused by members of the TNFR superfamily.</jats:p>","journal":"The Journal of Immunology","year":1998,"id":614223,"datarank":0.6190701577567639,"base_score":4.127134385045092,"endowment":4.127134385045092,"self_citation_contribution":0.6190701577567639,"citation_network_contribution":0.0,"self_endowment_contribution":0.6190701577567639,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":61,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1582715,"name":"Kaliannan Raju","orcid":null,"position":1,"is_corresponding":false},{"id":625572,"name":"Laszlo Radvanyi","orcid":"0000-0003-1603-3547","position":2,"is_corresponding":false},{"id":1582716,"name":"Yunping Lin","orcid":null,"position":3,"is_corresponding":false},{"id":1582717,"name":"Richard G Miller","orcid":null,"position":4,"is_corresponding":false},{"id":1582714,"name":"David Spaner","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"A Role for Perforin in Activation-Induced Cell Death","abstract":"<jats:title>Abstract</jats:title>\n                  <jats:p>The granule exocytosis pathway of T cell cytotoxicity is absent in mice whose perforin gene has been ablated by targeted mutagenesis. The ability of activated naive T cells to undergo apoptosis in vitro following reaggregation of the TCR complex with anti-TCR mAbs via a Fas-independent pathway was found to be defective in the absence of perforin. Protection from death was most marked in CD8+ T cells. In wild-type cells, perforin was expressed at the same time that apoptosis occurred, and blockade of perforin expression by either incubation with perforin antisense oligonucleotides or with anti-IL-2 Abs resulted in increased viability of activated T cells. The role of perforin was not via perforin-dependent fratricidal killing. The results suggest a model in which perforin acts internally to cause a form of activation-induced T cell death distinct from that caused by members of the TNFR superfamily.</jats:p>","is_dataset_classified":null,"base_score":4.127134385045092,"endowment":4.127134385045092,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"9510164","pmcid":null,"openalex_id":"https://openalex.org/W2100220782","authors":[],"funders":[],"total_grants":0,"fwci":2.0949,"citation_percentile":0.87888112,"influential_citations":0,"citation_trend":[{"year":2012,"count":3},{"year":2013,"count":1},{"year":2014,"count":1},{"year":2015,"count":2},{"year":2017,"count":1},{"year":2020,"count":1},{"year":2022,"count":1},{"year":2023,"count":1},{"year":2024,"count":2},{"year":2025,"count":2}],"oa_status":"bronze","license":"https://academic.oup.com/pages/standard-publication-reuse-rights","oa_locations":[{"url":"https://journals.aai.org/jimmunol/article-pdf/160/6/2655/1084214/im069802655o.pdf","host_type":"journal"},{"url":"https://journals.aai.org/jimmunol/article-pdf/160/6/2655/1084214/im069802655o.pdf","host_type":"publisher"},{"url":"https://academic.oup.com/jimmunol/article-pdf/160/6/2655/62180149/im069802655o.pdf","host_type":"publisher"},{"url":"https://doi.org/10.4049/jimmunol.160.6.2655","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/9510164","host_type":"repository"}],"fields_of_study":["Cell death mechanisms and regulation","Acute Lymphoblastic Leukemia research","NF-κB Signaling Pathways"],"mesh_terms":["Animals","Interleukin-2","Lymphocyte Activation","Membrane Glycoproteins","Mice, Inbred C57BL","T-Lymphocytes","Tumor Necrosis Factor-alpha","Cell Death","Mice, Knockout","fas Receptor","Mice","Pore Forming Cytotoxic Proteins","Perforin"],"keywords":["Perforin","Biology","Cell biology","Cytotoxic T cell","CD8","Programmed cell death","Apoptosis","T cell","Molecular biology","Immunology","In vitro","Antigen","Immune system","Genetics"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T11:40:58.848174Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}