{"doi":"10.4049/jimmunol.144.3.1037","title":"Functional studies of bovine alveolar neutrophils elicited with recombinant bovine IL-1 <i>beta</i>.","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Bovine rIL-1beta (rbIL-1beta) was instilled intrabronchially into the lungs of steers to elicit harvestable alveolar neutrophils for functional analysis. Before instillation, bronchoalveolar lavage samples from the steers consisted of 96.4 +/- 1.5% (mean +/- SEM) macrophages, with the remaining cells neutrophils and occasional lymphocytes. Four hours after instillation of 1.0 and 10.0 nmol of IL-1, the lavage samples consisted of 96.3 +/- 0.8% and 91.0 +/- 5.7% neutrophils, respectively. Alveolar neutrophils elicited with rbIL-1beta and challenged with the calcium ionophore, A23187, released similar amounts of leukotriene B4 (LTB4) and its nonenzymatic isomer LTB I, and significantly greater amounts of 5-hydroxyeicosatetraenoic acid and the nonenzymatic isomer LTB II, when compared with circulating neutrophils. The rbIL-1beta did not, by itself, stimulate release of arachidonate metabolites from circulating neutrophils in quantities that were detectable by HPLC. Circulating neutrophils, preincubated with rbIL-1beta and stimulated with A23187, released significantly greater amounts of 5-hydroxyeicosatetraenoic acid and total 5-lipoxygenase metabolites when compared with control cells not incubated with rbIL-1beta. Incubation of circulating neutrophils with rbIL-1beta and A23187 concurrently resulted in a significantly increased release of all 5-lipoxygenase metabolites of arachidonate. However, both the release of superoxide anion and bacterial killing by rbIL-1beta-elicited bovine alveolar neutrophils did not differ from the values obtained for circulating neutrophils.</jats:p>","journal":"The Journal of Immunology","year":1990,"id":45032,"datarank":0.7999786629001326,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"self_citation_contribution":0.32958368660043297,"citation_network_contribution":0.4703949762996997,"self_endowment_contribution":0.32958368660043297,"citer_contribution":0.4703949762996997,"corpus_percentile":null,"corpus_rank":null,"citation_count":8,"citer_count":7,"citers_with_citation_signal":6,"citers_with_endowment":6,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":210980,"name":"H M Sassenfeld","orcid":null,"position":1,"is_corresponding":false},{"id":210981,"name":"C R Maliszewdki","orcid":null,"position":2,"is_corresponding":false},{"id":210982,"name":"R M Silflow","orcid":null,"position":3,"is_corresponding":false},{"id":210983,"name":"P E Baker","orcid":null,"position":4,"is_corresponding":false},{"id":210984,"name":"S M Taylor","orcid":null,"position":5,"is_corresponding":false},{"id":210985,"name":"R W Leid","orcid":null,"position":6,"is_corresponding":false},{"id":210979,"name":"J R Heidel","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"base_score":2.1972245773362196,"endowment":2.1972245773362196,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"2153175","pmcid":null,"openalex_id":"https://openalex.org/W1938451090","authors":[],"funders":[{"funder_name":"NIAID NIH HHS","grant_id":"AI 07025","title":null}],"total_grants":1,"fwci":2.2791,"citation_percentile":0.87150075,"influential_citations":0,"citation_trend":[],"oa_status":"closed","license":"https://academic.oup.com/pages/standard-publication-reuse-rights","oa_locations":[{"url":"https://academic.oup.com/jimmunol/article-pdf/144/3/1037/62379548/1037.pdf","host_type":"publisher"},{"url":"https://doi.org/10.4049/jimmunol.144.3.1037","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/2153175","host_type":"repository"}],"fields_of_study":["Chronic Obstructive Pulmonary Disease (COPD) Research","Asthma and respiratory diseases","Immune Response and Inflammation","Biology","Medicine","Agricultural and Food Sciences"],"mesh_terms":["Animals","Arachidonate 5-Lipoxygenase","Arachidonic Acids","Blood Bactericidal Activity","Bronchoalveolar Lavage Fluid","Cattle","Dose-Response Relationship, Drug","Hydroxyeicosatetraenoic Acids","Interleukin-1","Neutrophils","Pulmonary Alveoli","Recombinant Proteins","Superoxides","Leukotrienes","Arachidonic Acid"],"keywords":["Leukotriene B4","Chemistry","Bronchoalveolar lavage","Hydroxyeicosatetraenoic acid","Pulmonary alveolus","Leukotriene","Incubation","BETA (programming language)","Neutrophile","Molecular biology","Arachidonic acid","Endocrinology","Internal medicine","Immunology","Biochemistry","In vitro","Biology","Inflammation","Enzyme","Lung","Medicine","Macrophage"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-02T00:03:13.507949Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}