{"doi":"10.4049/jimmunol.136.7.2348","title":"Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins.","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>A panel of antigen-specific mouse helper T cell clones was characterized according to patterns of lymphokine activity production, and two types of T cell were distinguished. Type 1 T helper cells (TH1) produced IL 2, interferon-gamma, GM-CSF, and IL 3 in response to antigen + presenting cells or to Con A, whereas type 2 helper T cells (TH2) produced IL 3, BSF1, and two other activities unique to the TH2 subset, a mast cell growth factor distinct from IL 3 and a T cell growth factor distinct from IL 2. Clones representing each type of T cell were characterized, and the pattern of lymphokine activities was consistent within each set. The secreted proteins induced by Con A were analyzed by biosynthetic labeling and SDS gel electrophoresis, and significant differences were seen between the two groups of T cell line. Both types of T cell grew in response to alternating cycles of antigen stimulation, followed by growth in IL 2-containing medium. Examples of both types of T cell were also specific for or restricted by the I region of the MHC, and the surface marker phenotype of the majority of both types was Ly-1+, Lyt-2-, L3T4+, Both types of helper T cell could provide help for B cells, but the nature of the help differed. TH1 cells were found among examples of T cell clones specific for chicken RBC and mouse alloantigens. TH2 cells were found among clones specific for mouse alloantigens, fowl gamma-globulin, and KLH. The relationship between these two types of T cells and previously described subsets of T helper cells is discussed.</jats:p>","journal":"The Journal of Immunology","year":1986,"id":9404,"datarank":19.6925236788501,"base_score":8.922658299524402,"endowment":8.922658299524402,"self_citation_contribution":1.3383987449286605,"citation_network_contribution":18.35412493392144,"self_endowment_contribution":1.3383987449286605,"citer_contribution":18.35412493392144,"corpus_percentile":90.9,"corpus_rank":2168,"citation_count":7499,"citer_count":190,"citers_with_citation_signal":190,"citers_with_endowment":190,"datacite_reuse_total":0,"is_dataset":false,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"1986-04-01","authors":[{"id":79331,"name":"H Cherwinski","orcid":null,"position":1,"is_corresponding":false},{"id":79332,"name":"M W Bond","orcid":null,"position":2,"is_corresponding":false},{"id":79333,"name":"M A Giedlin","orcid":null,"position":3,"is_corresponding":false},{"id":79334,"name":"R L Coffman","orcid":null,"position":4,"is_corresponding":false},{"id":79335,"name":"Timothy R. Mosmann","orcid":null,"position":5,"is_corresponding":false},{"id":79336,"name":"Holly Cherwinski","orcid":null,"position":6,"is_corresponding":false},{"id":79337,"name":"Martin Giedlin","orcid":null,"position":7,"is_corresponding":false},{"id":79338,"name":"Robert L. Coffman","orcid":null,"position":8,"is_corresponding":false},{"id":79330,"name":"T R Mosmann","orcid":null,"position":0,"is_corresponding":true}],"reference_count":3,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-03-01T18:20:47.508186Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}