{"doi":"10.4049/jimmunol.119.6.2084","title":"Surface Immunoglobulin and Fc Receptors on Murine B Lymphocytes: Loss of Receptor Interaction after Cell Activation","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Although surface immunoglobulin (sIg) and Fc receptors (FcR) are independent receptors in the membrane of B lymphocytes and are assumed not to interact under normal conditions, it has been shown that anti-Ig-induced redistribution, i.e., patching and capping, of sIg results in the co-capping of FcR, suggesting some type of receptor interaction. We have investigated this phenomenon by comparing the interaction of these receptors on normal vs activated B cells under a variety of conditions. It was found that, in contrast to unstimulated control cells, sIg and Fc receptors on blast cells induced by either Fab′2 anti-Ig or endotoxin protein do not interact, i.e., little if any co-capping was seen. This loss of receptor interaction was not due to increased cell size since cells which were stripped of their sIg and FcR and allowed to re-express both receptors for 24 hr, but which had not increased in size during this period, also failed to show co-capping behavior. Additional experiments showed that the Fc receptors on activated B cells could be capped directly by using complexes of FITC-KLH-anti-KLH, indicating that the loss of sIg-FcR co-capping on activated cells is not due to any restriction in the mobility of the Fc receptor in the membrane. These results strongly suggest that B lymphocyte activation is accompanied by membrane alterations that result in the loss of sIg and FcR interaction and that these changes do not require receptor redistribution and re-expression.</jats:p>","journal":"The Journal of Immunology","year":1977,"id":615198,"datarank":0.3596842909197557,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"self_citation_contribution":0.3596842909197557,"citation_network_contribution":0.0,"self_endowment_contribution":0.3596842909197557,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":10,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1585507,"name":"Howard L Weiner","orcid":null,"position":1,"is_corresponding":false},{"id":1585509,"name":"John W Moorhead","orcid":null,"position":2,"is_corresponding":false},{"id":1585506,"name":"Duncan J Scribner","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Surface Immunoglobulin and Fc Receptors on Murine B Lymphocytes: Loss of Receptor Interaction after Cell Activation","abstract":"<jats:title>Abstract</jats:title>\n               <jats:p>Although surface immunoglobulin (sIg) and Fc receptors (FcR) are independent receptors in the membrane of B lymphocytes and are assumed not to interact under normal conditions, it has been shown that anti-Ig-induced redistribution, i.e., patching and capping, of sIg results in the co-capping of FcR, suggesting some type of receptor interaction. We have investigated this phenomenon by comparing the interaction of these receptors on normal vs activated B cells under a variety of conditions. It was found that, in contrast to unstimulated control cells, sIg and Fc receptors on blast cells induced by either Fab′2 anti-Ig or endotoxin protein do not interact, i.e., little if any co-capping was seen. This loss of receptor interaction was not due to increased cell size since cells which were stripped of their sIg and FcR and allowed to re-express both receptors for 24 hr, but which had not increased in size during this period, also failed to show co-capping behavior. Additional experiments showed that the Fc receptors on activated B cells could be capped directly by using complexes of FITC-KLH-anti-KLH, indicating that the loss of sIg-FcR co-capping on activated cells is not due to any restriction in the mobility of the Fc receptor in the membrane. These results strongly suggest that B lymphocyte activation is accompanied by membrane alterations that result in the loss of sIg and FcR interaction and that these changes do not require receptor redistribution and re-expression.</jats:p>","is_dataset_classified":null,"base_score":2.3978952727983707,"endowment":2.3978952727983707,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"334982","pmcid":null,"openalex_id":"https://openalex.org/W1589947911","authors":[],"funders":[],"total_grants":0,"fwci":1.9001,"citation_percentile":0.85055421,"influential_citations":0,"citation_trend":[],"oa_status":"bronze","license":"https://academic.oup.com/pages/standard-publication-reuse-rights","oa_locations":[{"url":"https://journals.aai.org/jimmunol/article-pdf/119/6/2084/1004438/ji1190062084.pdf","host_type":"journal"},{"url":"https://journals.aai.org/jimmunol/article-pdf/119/6/2084/1004438/ji1190062084.pdf","host_type":"publisher"},{"url":"https://academic.oup.com/jimmunol/article-pdf/119/6/2084/62424543/ji1190062084.pdf","host_type":"publisher"},{"url":"https://doi.org/10.4049/jimmunol.119.6.2084","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/334982","host_type":"repository"},{"url":"https://mouseion.jax.org/ssbb1977/145","host_type":"repository"}],"fields_of_study":["Monoclonal and Polyclonal Antibodies Research","Cell Adhesion Molecules Research","T-cell and B-cell Immunology"],"mesh_terms":["Animals","B-Lymphocytes","Binding Sites, Antibody","Fluorescent Antibody Technique","Immunity, Cellular","Immunoglobulin Fc Fragments","Lymphocyte Activation","Mice, Inbred CBA","Rabbits","Receptors, Antigen, B-Cell","Mice"],"keywords":["Receptor","Cell surface receptor","Antibody","Surface Immunoglobulin","Cell membrane","Membrane","Lymphocyte","Cell biology","Cell","Fc receptor","Immunoglobulin Fc Fragments","Chemistry","Fragment crystallizable region","Immunoglobulin G","B cell","Biophysics","Biology","Molecular biology","Immunology","Biochemistry"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-08-02T19:16:52.382914Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}