{"doi":"10.4049/immunohorizons.2400059","title":"Chick Embryo Chorioallantoic Membrane as a Platform for Assessing the In Vivo Efficacy of Chimeric Antigen Receptor T-cell Therapy in Solid Tumors","abstract":"The fertilized chicken egg chorioallantoic membrane (CAM), a highly vascularized membrane nourishing the developing embryo, also supports rapid growth of three-dimensional vascularized tumors from engrafted cells and tumor explants. Because murine xenograft models suffer limitations of time, cost, and scalability, we propose CAM tumors as a rapid, efficient screening tool for assessing anti-tumor efficacy of chimeric Ag receptor (CAR) T cells against solid tumors. We tested the efficacy of human epidermal growth factor receptor 2 (HER2)-specific CAR T cells against luminescent, HER2-expressing (FaDu, SCC-47) or HER2-negative (MDA-MB-468) CAM-engrafted tumors. Three days after tumor engraftment, HER2-specific CAR T cells were applied to tumors grown on the CAM. Four days post-CAR T cell treatment, HER2-expressing FaDu and SCC-47 tumors treated with CAR T showed reduced viable cancer cells as assessed by luciferase activity. This reduction in viable tumor cells was confirmed by histology, with lower Ki-67 staining observed in CAR T cell-treated tumors relative to T cell-treated controls. Persistence of CAR T in CAM and tumor tissue 4 days post-treatment was confirmed by CD3 staining. Altogether, our findings support further development of the chick CAM as an in vivo system for rapid, scalable screening of CAR T cell efficacy against human solid tumors.","journal":"ImmunoHorizons","year":2024,"id":445421,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9609,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":748647,"name":"Emilie A. K. Warren","orcid":"0000-0003-2487-7213","position":1,"is_corresponding":false},{"id":1261345,"name":"Kershena S. Liao","orcid":"0000-0001-9839-5730","position":2,"is_corresponding":false},{"id":651508,"name":"Hsuan‐Chen Liu","orcid":"0000-0001-7857-0204","position":3,"is_corresponding":false},{"id":863474,"name":"Chieko Michikawa","orcid":"0000-0003-1181-5179","position":4,"is_corresponding":false},{"id":260507,"name":"Caroline E. Porter","orcid":"0000-0002-8500-2608","position":5,"is_corresponding":false},{"id":1261346,"name":"Gabrielle A. Wells","orcid":"0000-0003-3894-5037","position":6,"is_corresponding":false},{"id":1261347,"name":"Mariana Villanueva","orcid":"0000-0002-9476-5447","position":7,"is_corresponding":false},{"id":483185,"name":"Fábio Henrique Brasil da Costa","orcid":"0000-0002-2089-4051","position":8,"is_corresponding":false},{"id":486545,"name":"Ratna Veeramachaneni","orcid":"0000-0003-2515-0120","position":9,"is_corresponding":false},{"id":873533,"name":"Hugo Villanueva","orcid":"0000-0002-1845-3409","position":10,"is_corresponding":false},{"id":260513,"name":"Masataka Suzuki","orcid":"0000-0003-4086-4199","position":11,"is_corresponding":false},{"id":260512,"name":"Andrew G. Sikora","orcid":"0000-0002-8357-8435","position":12,"is_corresponding":false},{"id":972989,"name":"Allison Nipper","orcid":"0000-0003-3070-3439","position":0,"is_corresponding":true}],"reference_count":40,"raw_metadata":null,"created_at":"2026-07-19T02:01:42.089235Z","pmid":"39225630","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}