{"doi":"10.4049/immunohorizons.2000068","title":"Proteome-Wide Zika Virus CD4 T Cell Epitope and HLA Restriction Determination","abstract":"Zika virus (ZIKV) is a mosquito-borne pathogen that caused an epidemic in 2015-2016. ZIKV-specific T cell responses are functional in animal infection models, and helper CD4 T cells promote avid Abs in the vaccine context. The small volumes of blood available from field research limit the determination of T cell epitopes for complex microbes such as ZIKV. The goal of this project was efficient determination of human ZIKV CD4 T cell epitopes at the whole proteome scale, including validation of reactivity to whole pathogen, using small blood samples from convalescent time points when T cell response magnitude may have waned. Polyclonal enrichment of candidate ZIKV-specific CD4 T cells used cell-associated virus, documenting that T cells in downstream peptide analyses also recognize whole virus after Ag processing. Sequential query of bulk ZIKV-reactive CD4 T cells with pooled/single ZIKV peptides and molecularly defined APC allowed precision epitope and HLA restriction assignments across the ZIKV proteome and enabled discovery of numerous novel ZIKV CD4 T cell epitopes. The research workflow is useful for the study of emerging infectious diseases with a very limited human blood sample availability.","journal":"ImmunoHorizons","year":2020,"id":83574,"datarank":0.37273599746820013,"base_score":2.4849066497880004,"endowment":2.4849066497880004,"self_citation_contribution":0.37273599746820013,"citation_network_contribution":0.0,"self_endowment_contribution":0.37273599746820013,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":11,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9289,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":430026,"name":"LeAnn P. Nguyen","orcid":"0000-0002-9640-8902","position":1,"is_corresponding":false},{"id":430027,"name":"Elise Snoey","orcid":"0000-0001-9962-3336","position":2,"is_corresponding":false},{"id":294664,"name":"Christopher L. McClurkan","orcid":"0009-0008-0473-4366","position":3,"is_corresponding":false},{"id":430028,"name":"Kerry J. Laing","orcid":"0000-0001-9245-5325","position":4,"is_corresponding":false},{"id":431161,"name":"Lichun Dong","orcid":null,"position":5,"is_corresponding":false},{"id":55670,"name":"Alessandro Sette","orcid":"0000-0001-7013-2250","position":6,"is_corresponding":false},{"id":230241,"name":"Cecilia S. Lindestam Arlehamn","orcid":"0000-0001-7302-8002","position":7,"is_corresponding":false},{"id":431162,"name":"Danny M Altmann","orcid":null,"position":8,"is_corresponding":false},{"id":430029,"name":"Rosemary J. Boyton","orcid":"0000-0002-5608-0797","position":9,"is_corresponding":false},{"id":356427,"name":"Justin A. Roby","orcid":"0000-0003-2434-4273","position":10,"is_corresponding":false},{"id":108916,"name":"Michael Gale","orcid":"0000-0002-6332-7436","position":11,"is_corresponding":false},{"id":246884,"name":"Mars Stone","orcid":"0000-0001-5619-2767","position":12,"is_corresponding":false},{"id":36841,"name":"Michael P. Busch","orcid":"0000-0002-1446-125X","position":13,"is_corresponding":false},{"id":431163,"name":"Phillip Norris","orcid":null,"position":14,"is_corresponding":false},{"id":294672,"name":"David M. Koelle","orcid":"0000-0003-1255-9023","position":15,"is_corresponding":false},{"id":294663,"name":"Victoria Campbell","orcid":"0000-0001-6490-5974","position":0,"is_corresponding":true}],"reference_count":56,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T21:54:32.422022Z","pmid":"32753403","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}