{"doi":"10.4049/immunohorizons.2000011","title":"Bone Marrow–Derived Dendritic Cell Cultures from RAG−/− Mice Include IFN-γ–Producing NK Cells","abstract":"Abstract Dendritic cells (DCs) play a key role in the initiation of an immune response and are known as “professional” APCs because of their ability to activate naive T cells. A widely used method to generate DCs in vitro is to culture bone marrow (BM) cells or blood monocytes in the presence of GM-CSF and IL-4. In this study, we show that a small population of NK cells residing in the BM of RAG−/−, but not RAG−/− γc chain−/− mice, remain in the DC culture and is the source of IFN-γ produced after stimulation with LPS. These cells, which may represent early promoters of LPS-induced responses, have to be taken into account when interpreting experiments using BM-derived DCs.","journal":"ImmunoHorizons","year":2020,"id":106498,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9544,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":513629,"name":"L. Michael Thomas","orcid":"0000-0001-8372-9136","position":1,"is_corresponding":false},{"id":513630,"name":"Karen Laky","orcid":"0000-0002-8197-3941","position":2,"is_corresponding":false},{"id":418680,"name":"Mehrnoosh Abshari","orcid":"0000-0003-4217-0625","position":3,"is_corresponding":false},{"id":514290,"name":"Polly Matzinger","orcid":null,"position":4,"is_corresponding":false},{"id":513631,"name":"Eric O. Long","orcid":"0000-0002-7793-3728","position":5,"is_corresponding":false},{"id":513628,"name":"Kaveh Abdi","orcid":"0000-0002-9763-3757","position":0,"is_corresponding":true}],"reference_count":22,"raw_metadata":null,"created_at":"2026-07-18T23:12:27.124515Z","pmid":"32665300","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}