{"doi":"10.4014/jmb.2008.08058","title":"Effective Blocking of Microbial Transcriptional Initiation by dCas9-NG-Mediated CRISPR Interference","abstract":null,"journal":"Journal of Microbiology and Biotechnology","year":2020,"id":600290,"datarank":0.31191623125197543,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"self_citation_contribution":0.31191623125197543,"citation_network_contribution":0.0,"self_endowment_contribution":0.31191623125197543,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":7,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":150487,"name":"Hyun Ju Kim","orcid":null,"position":1,"is_corresponding":false},{"id":1538921,"name":"Sang Jun Lee","orcid":null,"position":2,"is_corresponding":false},{"id":1538920,"name":"Bumjoon Kim","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Effective Blocking of Microbial Transcriptional Initiation by dCas9-NG-Mediated CRISPR Interference","abstract":"transcripts confirmed that dCas9-NG can completely repress the promoter by recognizing DNA targets with PAM of 5'-NGG, NGA, NGC, NGT, and NAG. Our study showed possible PAM sequences for dCas9-NG and provided information on target-specific sgRNA design for regulation of both gene expression and cellular metabolism.","is_dataset_classified":null,"base_score":2.0794415416798357,"endowment":2.0794415416798357,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"32958732","pmcid":"PMC9728369","openalex_id":"https://openalex.org/W3087027348","authors":[],"funders":[{"funder_name":"National Research Foundation of Korea","grant_id":"NRF-2019R1A4A1024764","title":null},{"funder_name":"National Research Foundation of Korea","grant_id":"2018R1A6A3A11051083","title":null},{"funder_name":"National Research Foundation of Korea","grant_id":"2017R1E1A1A01075124","title":null},{"funder_name":"CJ CheilJedang Institute of Biotechnology","grant_id":"CG-20-17-01-0002","title":null}],"total_grants":4,"fwci":0.4068,"citation_percentile":0.59611373,"influential_citations":0,"citation_trend":[{"year":2021,"count":3},{"year":2023,"count":2},{"year":2024,"count":1},{"year":2025,"count":1}],"oa_status":"bronze","license":"cc-by","oa_locations":[{"url":"https://www.jmb.or.kr/journal/download_pdf.php?doi=10.4014/jmb.2008.08058","host_type":"journal"},{"url":"https://www.jmb.or.kr/journal/download_pdf.php?doi=10.4014/jmb.2008.08058","host_type":"publisher"},{"url":"https://pdf.medrang.co.kr/JMB2/2020/030/JMB030-12-16.pdf","host_type":"publisher"},{"url":"https://doi.org/10.4014/jmb.2008.08058","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/32958732","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/9728369","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC9728369","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC9728369?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["CRISPR and Genetic Engineering","RNA and protein synthesis mechanisms","RNA Interference and Gene Delivery"],"mesh_terms":["RNA, Guide, CRISPR-Cas Systems","DNA","Escherichia coli","Galactose","Mutation","Promoter Regions, Genetic","Gene Expression","RNA, Guide, Kinetoplastida","Clustered Regularly Interspaced Short Palindromic Repeats","CRISPR-Cas Systems"],"keywords":["Cas9","CRISPR","Guide RNA","CRISPR interference","Biology","DNA","Transcription (linguistics)","Gene","Subgenomic mRNA","RNA","RNA interference","Computational biology","Cell biology","Molecular biology","Genetics","D-galactose","Pam Sequence","Dcas9-ng","Gal Promoter"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T12:43:27.679964Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}