{"doi":"10.4014/jmb.1211.11009","title":"Production of Chlorphenesin Galactoside by Whole Cells of β-Galactosidase-Containing Escherichia coli","abstract":null,"journal":"Journal of Microbiology and Biotechnology","year":2013,"id":607768,"datarank":0.38474240361923057,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"self_citation_contribution":0.38474240361923057,"citation_network_contribution":0.0,"self_endowment_contribution":0.38474240361923057,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1560694,"name":"Sang-Eun Lee","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Production of Chlorphenesin Galactoside by Whole Cells of β-Galactosidase-Containing Escherichia coli","abstract":"We investigated the transgalactosylation reaction of chlorphenesin (CPN) using β-galactosidase (β-gal)-containing Escherichia coli (E. coli) cells, in which galactose from lactose was transferred to CPN. The optimal CPN concentration for CPN galactoside (CPN-G) synthesis was observed at 40 mM under the conditions that lactose and β-gal (as E. coli cells) were 400 g/l and 4.8 U/ml, respectively, and the pH and temperature were 7.0 and 40oC, respectively. The time-course profile of CPN-G synthesis under these optimal conditions showed that CPN-G synthesis from 40 mM CPN reached a maximum of about 27 mM at 12 h. This value corresponded to an about 67% conversion of CPN to CPN-G, which was 4.47-5.36-fold higher than values in previous reports. In addition, we demonstrated by thin-layer chromatography to detect the sugar moiety that galactose was mainly transferred from lactose to CPN. Liquid chromatography-mass spectrometry revealed that CPN-G and CPN-GG (CPN galactoside, which accepted two galactose molecules) were definitively identified as the synthesized products using β-gal-containing E. coli cells. In particular, because we did not use purified β-gal, our β-gal-containing E. coli cells might be practical and cost-effective for enzymatically synthesizing CPN-G. It is expected that the use of β-gal-containing E. coli will be extended to galactose derivatization of other drugs to improve their functionality.","is_dataset_classified":null,"base_score":2.5649493574615367,"endowment":2.5649493574615367,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"23676917","pmcid":null,"openalex_id":"https://openalex.org/W1993200409","authors":[],"funders":[],"total_grants":0,"fwci":0.6879,"citation_percentile":0.68339751,"influential_citations":0,"citation_trend":[{"year":2014,"count":1},{"year":2015,"count":2},{"year":2016,"count":2},{"year":2019,"count":1},{"year":2020,"count":1},{"year":2021,"count":1},{"year":2023,"count":2},{"year":2025,"count":1},{"year":2026,"count":1}],"oa_status":"closed","license":null,"oa_locations":[{"url":"https://pdf.medrang.co.kr/JMB2/2013/023/JMB023-06-12_FDOC_1.pdf","host_type":"publisher"},{"url":"https://doi.org/10.4014/jmb.1211.11009","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/23676917","host_type":"repository"}],"fields_of_study":["Enzyme Catalysis and Immobilization","Pharmacogenetics and Drug Metabolism","Plant biochemistry and biosynthesis","Biotransformation","Chlorphenesin","Chromatography, Liquid","Chromatography, Thin Layer","Enzyme Stability","Escherichia coli","Galactose","Hydrogen-Ion Concentration","Lactose","Mass Spectrometry","Metabolic Networks and Pathways","Temperature","beta-Galactosidase"],"mesh_terms":["beta-Galactosidase","Biotransformation","Chlorphenesin","Chromatography, Liquid","Chromatography, Thin Layer","Enzyme Stability","Escherichia coli","Galactose","Hydrogen-Ion Concentration","Lactose","Mass Spectrometry","Temperature","Metabolic Networks and Pathways"],"keywords":["Lactose","Galactoside","Escherichia coli","Galactose","Chemistry","Derivatization","Beta-galactosidase","Biochemistry","Chromatography","Mass spectrometry","Enzyme"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-30T06:58:16.920919Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}