{"doi":"10.3892/etm.2020.8574","title":"miR‑106b‑5p promotes cell proliferation and cell cycle progression by directly targeting CDKN1A in osteosarcoma","abstract":null,"journal":"Experimental and Therapeutic Medicine","year":2020,"id":599929,"datarank":0.42498200160843247,"base_score":2.833213344056216,"endowment":2.833213344056216,"self_citation_contribution":0.42498200160843247,"citation_network_contribution":0.0,"self_endowment_contribution":0.42498200160843247,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":16,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":709802,"name":"Hongwei Chen","orcid":"0000-0003-3672-2876","position":1,"is_corresponding":false},{"id":418652,"name":"Yan Liu","orcid":"0000-0002-8581-2571","position":2,"is_corresponding":false},{"id":88604,"name":"Xiaolin Li","orcid":"0000-0002-3368-159X","position":3,"is_corresponding":false},{"id":1537749,"name":"Chaoju Zhang","orcid":null,"position":4,"is_corresponding":false},{"id":1537753,"name":"Qunyan Qin","orcid":null,"position":5,"is_corresponding":false},{"id":1537755,"name":"Qixiong Pang","orcid":null,"position":6,"is_corresponding":false},{"id":814162,"name":"Chuan He","orcid":"0000-0002-8532-2898","position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"miR‑106b‑5p promotes cell proliferation and cell cycle progression by directly targeting CDKN1A in osteosarcoma","abstract":"MicroRNA (miR)-106b-5p has been reported to act as both an oncogene and tumor suppressor in several tumors. The aim of the present study was to investigate the biological function of miR-106b-5p in osteosarcoma (OS). miR-106b-5p expression was observed to be significantly increased in OS tissues and cell lines. MTT assay and flow cytometry analysis determined that miR-106b-5p inhibitor transfection suppressed OS cell proliferation and induced cell cycle G0/G1 phase arrest. Furthermore, bioinformatics analysis and a luciferase reporter assay demonstrated that cyclin-dependent kinase inhibitor 1A (CDKN1A) was a potential target of miR-106b-5p. p21 protein expression was found to be significantly increased by miR-106b-5p downregulation in OS cells. Further analysis demonstrated that CDKN1A was downregulated in OS tissues and was negatively correlated with miR-106b-5p expression. Furthermore, upregulation of CDKN1A expression mimicked, whilst CDKN1A knockdown reversed the suppressive effects of miR-106b-5p inhibitor on OS cell proliferation and cell cycle progression. In summary, the present data suggested that miR-106b-5p promotes cell proliferation and cell cycle progression by directly targeting CDKN1A in OS.","is_dataset_classified":null,"base_score":2.833213344056216,"endowment":2.833213344056216,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"32266016","pmcid":"PMC7132225","openalex_id":"https://openalex.org/W3009790915","authors":[],"funders":[],"total_grants":0,"fwci":0.8762,"citation_percentile":0.68178419,"influential_citations":0,"citation_trend":[{"year":2020,"count":1},{"year":2021,"count":2},{"year":2022,"count":5},{"year":2023,"count":3},{"year":2024,"count":2},{"year":2025,"count":3}],"oa_status":"gold","license":"cc-by-nc-nd","oa_locations":[{"url":"https://www.spandidos-publications.com/10.3892/etm.2020.8574/download","host_type":"journal"},{"url":"https://www.spandidos-publications.com/10.3892/etm.2020.8574/download","host_type":"publisher"},{"url":"https://doi.org/10.3892/etm.2020.8574","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/32266016","host_type":"repository"},{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/7132225","host_type":"repository"},{"url":"https://europepmc.org/articles/PMC7132225","host_type":"Europe_PMC"},{"url":"https://europepmc.org/articles/PMC7132225?pdf=render","host_type":"Europe_PMC"}],"fields_of_study":["MicroRNA in disease regulation","Circular RNAs in diseases","Cancer-related molecular mechanisms research"],"mesh_terms":[],"keywords":["Cell cycle","Cell growth","Oncogene","Cancer research","microRNA","Downregulation and upregulation","Biology","Flow cytometry","Cell","Cell cycle checkpoint","Osteosarcoma","Transfection","Cyclin D1","MTT assay","Gene knockdown","Cell culture","Cell biology","Chemistry","Molecular biology","G0/g1 Phase","Cyclin Dependent Kinase Inhibitor 1A","Microrna-106b-5p"],"sdg_mappings":[],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[{"name":"doi"}],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-29T11:42:24.951905Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}