{"doi":"10.3855/jidc.5866","title":"Retrospective screening of acute undifferentiated fever serum samples with universal flavivirus primers","abstract":"<jats:p>Introduction: Fever is a common symptom of many tropical diseases and in many cases the etiologic agent remains unidentified as a consequence of either the etiologic agent not being part of routine diagnostic screening or as a consequence of false negatives on standard diagnostic tests. Methodology: This study screened a well characterized panel of 274 serum samples collected on day of admission from adult patients with acute undifferentiated fever admitted to a hospital in Nakhon Ratchasima, Thailand by RT-PCR using pan-flavivirus degenerate primers. Results: Subsequent clinical diagnosis was achieved for 38 of the patients, and included 19 cases of dengue fever. RT-PCR screening identified seven positive samples (2.5%) which were revealed by sequence analysis to be dengue virus 1 (2 cases), dengue virus 2 (2 cases) and dengue virus 3 (3 cases). Only 5 out of 19 (26%) serum samples from patients subsequently diagnosed with dengue were positive, but 2 samples which clinically remained undiagnosed were shown to be positive for dengue virus. Sequence analysis suggested that the dengue virus 3 cases occurred as a result of importation of a strain of dengue from India or China. No other flaviviruses were identified. Conclusions: No evidence was found of other flaviviruses besides dengue circulating in this population. Despite improved diagnostic tests, cases of dengue are still evading correct diagnosis.</jats:p>","journal":"The Journal of Infection in Developing Countries","year":2015,"id":591023,"datarank":0.18527809180477384,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"self_citation_contribution":0.16479184330021646,"citation_network_contribution":0.02048624850455737,"self_endowment_contribution":0.16479184330021646,"citer_contribution":0.02048624850455737,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":2,"citers_with_citation_signal":2,"citers_with_endowment":2,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":null,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":null,"fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1512121,"name":"Sirikwan Libsittikul","orcid":null,"position":1,"is_corresponding":false},{"id":138211,"name":"Sutee Yoksan","orcid":null,"position":2,"is_corresponding":false},{"id":138001,"name":"Prasert Auewarakul","orcid":null,"position":3,"is_corresponding":false},{"id":316981,"name":"Yupin Suputtamongkol","orcid":"0000-0001-7324-1698","position":4,"is_corresponding":false},{"id":138003,"name":"Duncan R. Smith","orcid":"0000-0002-6592-9852","position":5,"is_corresponding":false},{"id":1512120,"name":"Sarawut Khongwichit","orcid":null,"position":0,"is_corresponding":false}],"reference_count":0,"raw_metadata":{"has_enrichment":true,"resolved":true,"title":"Retrospective screening of acute undifferentiated fever serum samples with universal flavivirus primers","abstract":"<jats:p>Introduction: Fever is a common symptom of many tropical diseases and in many cases the etiologic agent remains unidentified as a consequence of either the etiologic agent not being part of routine diagnostic screening or as a consequence of false negatives on standard diagnostic tests. Methodology: This study screened a well characterized panel of 274 serum samples collected on day of admission from adult patients with acute undifferentiated fever admitted to a hospital in Nakhon Ratchasima, Thailand by RT-PCR using pan-flavivirus degenerate primers. Results: Subsequent clinical diagnosis was achieved for 38 of the patients, and included 19 cases of dengue fever. RT-PCR screening identified seven positive samples (2.5%) which were revealed by sequence analysis to be dengue virus 1 (2 cases), dengue virus 2 (2 cases) and dengue virus 3 (3 cases). Only 5 out of 19 (26%) serum samples from patients subsequently diagnosed with dengue were positive, but 2 samples which clinically remained undiagnosed were shown to be positive for dengue virus. Sequence analysis suggested that the dengue virus 3 cases occurred as a result of importation of a strain of dengue from India or China. No other flaviviruses were identified. Conclusions: No evidence was found of other flaviviruses besides dengue circulating in this population. Despite improved diagnostic tests, cases of dengue are still evading correct diagnosis.</jats:p>","is_dataset_classified":null,"base_score":1.0986122886681096,"endowment":1.0986122886681096,"datacite_reuse_total":0,"file_count":0,"downloads":0,"views":0,"has_version_chain":false,"is_dataset":false,"is_oa":false,"pmid":"26230127","pmcid":null,"openalex_id":"https://openalex.org/W1792837961","authors":[],"funders":[],"total_grants":0,"fwci":0.0,"citation_percentile":0.04979445,"influential_citations":0,"citation_trend":[{"year":2023,"count":2}],"oa_status":"gold","license":"cc-by","oa_locations":[{"url":"https://jidc.org/index.php/journal/article/download/26230127/1345","host_type":"journal"},{"url":"https://jidc.org/index.php/journal/article/download/26230127/1345","host_type":"publisher"},{"url":"https://doi.org/10.3855/jidc.5866","host_type":"journal"},{"url":"https://pubmed.ncbi.nlm.nih.gov/26230127","host_type":"repository"},{"url":"https://doaj.org/article/e3fa9407e7ec4363836dcb8bccdcc995","host_type":"repository"}],"fields_of_study":["Mosquito-borne diseases and control","Viral Infections and Outbreaks Research","Biological Research and Disease Studies","Adult","DNA Primers","Dengue Virus","Fever of Unknown Origin","Flaviviridae Infections","Hospitals","Humans","Mass Screening","Prospective Studies","Retrospective Studies","Reverse Transcriptase Polymerase Chain Reaction","Serum","Thailand"],"mesh_terms":["Adult","Dengue Virus","Fever of Unknown Origin","Hospitals","Humans","Mass Screening","Prospective Studies","Retrospective Studies","Thailand","DNA Primers","Flaviviridae Infections","Reverse Transcriptase Polymerase Chain Reaction","Serum"],"keywords":["Dengue fever","Dengue virus","Flavivirus","Virology","Medicine","Flaviviridae","Virus","Population","Viral disease","Immunology","Environmental health"],"sdg_mappings":[{"sdg_number":0,"sdg_label":"Good health and well-being"}],"linked_datasets":[],"clinical_trials":[],"software_tools":[],"database_accessions":[],"source":"live","citation_network_status":"fetched"},"created_at":"2026-07-25T13:49:03.907370Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}