{"doi":"10.3791/65114","title":"Sonodynamic Therapy for the Treatment of Glioblastoma Multiforme in a Mouse Model Using a Portable Benchtop Focused Ultrasound System","abstract":"Sonodynamic therapy (SDT) is an application of focused ultrasound (FUS) that enables a sonosensitizing agent to prime tumors for increased sensitivity during sonication. Unfortunately, current clinical treatments for glioblastoma (GBM) are lacking, leading to low long-term survival rates among patients. SDT is a promising method for treating GBM in an effective, noninvasive, and tumor-specific manner. Sonosensitizers preferentially enter tumor cells compared to the surrounding brain parenchyma. The application of FUS in the presence of a sonosensitizing agent generates reactive oxidative species resulting in apoptosis. Although this therapy has been shown previously to be effective in preclinical studies, there is a lack of established standardized parameters. Standardized methods are necessary to optimize this therapeutic strategy for preclinical and clinical use. In this paper, we detail the protocol to perform SDT in a preclinical GBM rodent model using magnetic resonance-guided FUS (MRgFUS). MRgFUS is an important feature of this protocol, as it allows for specific targeting of a brain tumor without the need for invasive surgeries (e.g., craniotomy). The benchtop device used here can focus on a specific location in three dimensions by clicking on a target on an MRI image, making target selection a straightforward process. This protocol will provide researchers with a standardized preclinical method for MRgFUS SDT, with the added flexibility to change and optimize parameters for translational research.","journal":"Journal of Visualized Experiments","year":2023,"id":366732,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":5,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9573,"is_data_producer":false,"deposit_databanks":null,"is_oa":false,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1123406,"name":"Taylor Anderson","orcid":"0000-0003-1362-8414","position":1,"is_corresponding":false},{"id":675787,"name":"Shivani Kapoor","orcid":"0009-0005-1213-2312","position":2,"is_corresponding":false},{"id":928456,"name":"Rasika Thombre","orcid":null,"position":3,"is_corresponding":false},{"id":1123900,"name":"Ruixing Liang","orcid":null,"position":4,"is_corresponding":false},{"id":1123407,"name":"Emre Derin","orcid":"0009-0001-5309-8158","position":5,"is_corresponding":false},{"id":928457,"name":"Kelley M. Kempski-Leadingham","orcid":null,"position":6,"is_corresponding":false},{"id":285790,"name":"Santosh K. Yadav","orcid":"0000-0001-9728-4561","position":7,"is_corresponding":false},{"id":326901,"name":"Betty Tyler","orcid":"0000-0001-9800-6969","position":8,"is_corresponding":false},{"id":539658,"name":"Amir Manbachi","orcid":"0000-0003-0206-5230","position":9,"is_corresponding":false},{"id":928455,"name":"Griffin Mess","orcid":null,"position":0,"is_corresponding":true}],"reference_count":9,"raw_metadata":null,"created_at":"2026-07-19T01:14:59.372548Z","pmid":"36847383","pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}